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化学指导的单细胞转录组学揭示了在securinine生物合成中的硫转移酶介导的支架重塑
Sungjun Choung1, Gyumin Kang2, Taein Kim1
1Department of Biological Sciences, KAIST, Daejeon, Republic of Korea.
Nature communications
|January 23, 2026
概括
研究人员阐明了Flueggea suffruticosa.中securinega类化合物的生物合成. 确定了关键的酶,揭示了硫转移酶.
科学领域:
- 自然产品生物合成 自然产品生物合成
- 植物生物化学 植物生物化学
- 分子生物学分子生物学
背景情况:
- 塞库里尼加类化合物具有独特的四环结构,具有药物相关性.
- 这些类化合物的生物合成在Flueggea suffruticosa中尚未完全理解.
研究的目的:
- 为了阐明Flueggea suffruticosa中securinega类化合物的核心生物合成途径.
- 识别关键的酶和中间体,参与形成securinin和相关化合物.
主要方法:
- 使用稳定同位素标记的养实验.
- 单细胞转录组学与化学逻辑结合使用.
- 酶活动通过生物化学测试来表征.
主要成果:
- 鉴定出一种减少酶 (FsMS),它可以将前列尼斯达URILIDE转化为半列尼斯达URILIDE.
- 鉴定出了硫转移酶 (FsNSST1和FsNSST2),它们催化了新苏里南的O-硫化.
- 素酸乙的neosecurinanes自发地重新排列形成安全,揭示了硫转移酶在脚手架重塑中的新角色.
- 发现生物合成基因在与血管相关的细胞中被共同表达.
结论:
- 这项研究揭示了一种新的生物合成途径,用于securinega类化合物,包括securinine.
- 硫转移酶在化物生物合成期间调解支架重塑方面发挥着至关重要的,意想不到的作用.
- 化学指导的单细胞转录组学是一种强大的方法,用于剖析复杂的植物生物合成途径.
- 在特定细胞类型中识别共同表达的基因有助于优先考虑未来的研究候选者.
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