在治疗老鼠中永久性中脑动脉阻塞损伤时,减拉聚合物的时间依赖性有效性
Olga A Bragina1, Jun Wu2, Zefeng Peng2
1Lovelace Biomedical Research Institute, Albuquerque, NM, USA.
减拉聚合物 (DRPs) 在大鼠缺血性中风模型中有效改善了微血管大脑血流和氧气输送. 即使在中风发作后数小时内服用,DRPs也显示出显著的治疗益处.
科学领域:
- 神经科学是一个神经科学.
- 生物医学工程 生物医学工程
- 药理学 药理学是指药理学的学科.
背景情况:
- 缺血性中风显著损害了微血管大脑血流 (mvCBF),阻碍了组织的氧化.
- 目前的中风治疗方法往往忽略了mvCBF在氧气输送中的关键作用.
- 之前的研究表明,阻力降低聚合物 (DRPs) 可以恢复mvCBF和中风后的氧化.
研究的目的:
- 评估在永久性中脑动脉封闭 (pMCAO) 后在不同时间点服用DRP的疗效.
- 评估延迟DRP给药对大脑微循环和组织缺氧的影响.
主要方法:
- 鼠接受了pMCAO,并在0.5,3或6小时后接受静脉注射DRPs (2μg/ml) 或盐水.
- 激光光斑对比成像 (LSCI) 评估了区域大脑血流和缺血区域.
- 在体内2光子激光扫描显微镜 (2PLSM) 评估了mvCBF和组织缺氧 (NADH).
主要成果:
- pMCAO逐渐减少皮质mvCBF和诱导组织缺氧.
- 与盐水相比,在pMCAO后0.5小时,3小时和6小时服用DRP显著改善了大脑微循环和氧气输送.
- 虽然效果随着延迟服用而下降,但DRPs在中风后6小时内仍然具有显著的有效性.
结论:
- 药物增强剂通过膜和皮膜瘤增强附带微循环流.
- DRPs代表了对缺血性中风的一种新且有效的辅助疗法.
- 延迟DRP的使用显示了治疗潜力,即使没有再注血.
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