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生物信息学分析和人口样本验证冠状动脉心脏病中与铁化相关的基因:一种病例对照研究
Li-Heng Li1, Cui-Hua Chen1, Qing-Hua Dong2
1Department of Anesthesiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, People's Republic of China.
Medicine
|January 24, 2026
概括
这项研究将细胞死亡过程铁亡与冠心病 (CHD) 联系起来. 研究人员确定NOX4是CHD患者表达升高的关键基因,表明其作为诊断生物标志物的潜力.
科学领域:
- 生物医学研究的研究.
- 遗传学 是一个遗传学.
- 心血管疾病是什么心血管疾病
背景情况:
- 冠状动脉心脏病 (CHD) 构成了严重的健康负担.
- 铁亡是一种受调节的细胞死亡形式,越来越多地与各种病理有关.
- 铁化在冠状动脉疾病发病过程中的特定作用仍然不完全理解.
研究的目的:
- 为了调查铁亡和冠心病 (CHD) 之间的关联.
- 使用生物信息学识别与心脏病中铁性相关的基因和潜在的诊断生物标志物.
- 在患者样本中验证已识别的生物标志物.
主要方法:
- 对CHD基因表达数据集的生物信息学分析 (GSE23561).
- 集成与来自FerrDb数据库的与ferroptosis相关的基因.
- 基因本体学,KEGG途径和疾病本体学丰富分析.
- 生物标志物选择的 LASSO 物流回归.
- 进行元分析和RT-PCR验证.
主要成果:
- 在心血管疾病中确定了122个与铁化相关的基因,突出了氧化应激和细胞膜功能的作用.
- 拉索回归确定了PRKAA2,NOX4,GLS2和G6PD作为潜在的诊断生物标志物.
- 在心血管疾病患者中观察到NOX4表达显著上调 (P<.05).
- 通过RT-PCR验证了CHD患者样本中NOX4的增加表达.
- NOX4上调可能与冠状动脉样硬化和心肌损伤有关.
结论:
- 铁死在冠状动脉心脏病的发病过程中发挥着作用.
- NOX4是冠心病的一个有前途的诊断生物标志物,在受影响的个体中表达高.
- 这些发现提供了关于CHD机制的见解,以及早期诊断和治疗策略的潜在途径.
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