淋巴体器官在非肥胖的糖尿病戈托-卡基扎基老鼠中重塑,这些老鼠被SARS-CoV-2抗原免疫
Sara Araujo Pereira1,2, Mariana Cruz Lazzarin1,3, Maria Lucia Zaidan Dagli4
1Laboratory of Pathophysiology, Butantan Institute, São Paulo, Brazil.
概括
2型糖尿病 (T2DM) 影响免疫反应. 这项对非肥胖糖尿病大鼠的研究表明,T2DM改变了淋巴细胞器官结构,可能增强了对SARS-CoV-2抗原的抗体产生.
科学领域:
- 免疫学 免疫学 免疫学
- 内分泌学 在内分泌学.
- 病理学 病理学 病理学
背景情况:
- 2型糖尿病 (T2DM) 与较差的COVID-19结果和降低疫苗疗效有关.
- 除了肥胖,T2DM对免疫反应和淋巴细胞器官结构的独立影响尚不清楚.
研究的目的:
- 调查T2DM对抗体产生和淋巴细胞器官形态学的孤立影响.
- 在SARS-CoV-2抗原免疫接种后的非肥胖T2DM大鼠模型 (Goto-Kakizaki大鼠) 中评估免疫反应和组织结构.
主要方法:
- 戈托-卡基扎基 (GK) 和威斯塔尔 (对照) 鼠被用两种不同的SARS-CoV-2抗原配方免疫.
- 使用ELISA测量了抗体 (anti-Spike IgG) 标位.
- 进行了淋巴体器官 (胸腺,脏,中腔淋巴结) 的组织学和形态学分析.
主要成果:
- 与对照大鼠相比,GK大鼠表现出明显更高的抗尖峰IgG标位.
- 在GK大鼠的胸腺 (内卷) 和脏 (白肉质重塑,生殖中心扩大,边缘区域减少) 中观察到形态变化.
- 中腔淋巴结在GK和对照大鼠中表现出类似的形态.
结论:
- 在GK大鼠中,非肥胖的T2DM与对SARS-CoV-2抗原的增强抗体反应有关.
- 在初级和二级淋巴体器官中,T2DM会诱导明显的结构重塑.
- 这些淋巴细胞器官的变化可能有助于在免疫挑战期间观察到的抗体生产变化.
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