相关实验视频

Updated: Jan 25, 2026

Preparation of High-Temperature Sample Grids for Cryo-EM
05:05

Preparation of High-Temperature Sample Grids for Cryo-EM

Published on: July 26, 2021

4.2K

有效和快速的隔离本地AMPA受体复合体的冷-EM

Jumi Park1, Eric Gouaux1,2

  • 1Vollum Institute, Oregon Health & Science University, Portland, Oregon, USA.

Protein science : a publication of the Protein Society
|January 24, 2026
PubMed
概括

研究人员使用整个大脑组织迅速分离出原生AMPA受体 (AMPAR) 和它们相关的蛋白质. 这种方法揭示了用于结构研究的AMPAR复合物的新组成和构造.

关键词:
在AMPA受体上,AMPA受体是化-EMEM可以使用.原生膜蛋白质原生膜蛋白质快速隔离 快速隔离

更多相关视频

A High-content Assay for Monitoring AMPA Receptor Trafficking10:34

A High-content Assay for Monitoring AMPA Receptor Trafficking

Published on: January 28, 2019

8.0K
Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

5.9K

相关概念视频

Internal Receptors01:31

Internal Receptors

Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
74.3K
Interaction of EM Radiation with Matter: Spectroscopy01:12

Interaction of EM Radiation with Matter: Spectroscopy

Electromagnetic (EM) radiation can be considered an oscillating electric and magnetic field propagating through a medium that can interact with matter in its path. The electric field in the radiation can interact with electrical charges in the atoms or molecules in the matter. On the other hand, the magnetic field can interact with the magnetic field in the atomic nucleus. The study of the interaction between electromagnetic radiation and matter is termed spectroscopy. Spectroscopy is the study...
3.1K
Receptor-mediated Endocytosis01:39

Receptor-mediated Endocytosis

Overview
110.7K
Dual Nature of Electromagnetic (EM) Radiation01:10

Dual Nature of Electromagnetic (EM) Radiation

Electromagnetic (EM) radiation consists of electric and magnetic field components oscillating in planes perpendicular to each other and mutually perpendicular to radiation propagation through space. EM radiation can be classified as a wave, characterized by the properties of waves such as wavelength (denoted as λ) and frequency (represented by ν).
Wavelength is the distance between two consecutive peaks (the highest point) or troughs (the lowest point) in the wave. Frequency is the number of...
3.7K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.2K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
86.3K