开发和验证LC-MS/MS方法,用于在蔬菜中确定多种残留农药
Renata de Oliveira Silva1, Amanda Fonseca Lopes2, Crisiana de Andrade Nobre1
1Núcleo de Tecnologia e Qualidade Industrial do Ceará, Fortaleza CEP:60.440-552, Ceara, Brazil.
Food chemistry
|January 24, 2026
概括
一种新的液态色谱-并联质谱法准确检测到蔬菜中的17种农药. 在西兰花中发现的酸盐低于欧盟限制,确保了食品安全.
科学领域:
- 农业化学 农业化学
- 分析化学 分析化学
- 食品安全 食品安全
背景情况:
- 蔬菜中的农药残留物对人类健康构成风险.
- 准确的检测方法对于监控遵守法规限制的监控至关重要.
研究的目的:
- 开发和验证一种敏感的分析方法,用于确定花和西兰花中的17种农药.
- 来自巴西的商业蔬菜样本中评估农药残留物的存在.
主要方法:
- 液体色谱与双重质谱 (LC-MS/MS) 使用电喷射电离 (ESI).
- 使用 Quenchers 方法进行提取.
- 方法验证包括选择性,线性,LOD,LOQ,精度和准确性.
主要成果:
- LC-MS/MS方法表现出良好的灵敏度,该方法的检测极限 (LODm) 为2.0μg kg-1.1.
- 大多数分析品的高精度 (≤20%) 和准确性 (70-120%) 得到了实现.
- 在西兰花样本中检测到亚酸的含量低于欧盟最大残留量限制 (MRL).
结论:
- 开发的LC-MS/MS方法适用于可靠量化17种花和西兰花中的农药.
- 结果表明,分析的蔬菜样本符合已确定的农药残留指南.
- 这种方法支持监管监测,并确保消费蔬菜的安全.
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