来自1-尼特罗皮林诱导的THP-1泡细胞的条件介质促进了肺上皮细胞中的前瘤发生
Ankita Bagde1, Atul Katarkar2, K Krishnamurthi1
1Waste and Chemical Toxicity Assessment, CSIR-National Environmental Engineering Research Institute, Nagpur 440020, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
概括
柴油废气成分1-尼托烯 (1NP) 通过酸受体 (AhR) 激活在巨细胞中触发泡细胞的形成. 这一过程通过创造支持瘤的微环境,促进了肺癌的进展.
科学领域:
- 环境健康 环境健康
- 毒理学 毒理学 毒理学
- 癌症生物学 癌症生物学
背景情况:
- 柴油废气 (DE) 是一个重要的环境污染物.
- 一个DE成分的1-基 (1NP) 与氧化应激和DNA损伤有关.
- 1NP在膜巨细胞功能障碍和肺癌发生中的作用尚不清楚.
研究的目的:
- 研究1NP在巨细胞泡细胞形成中的作用.
- 阐明1NP诱导的泡细胞形成的机制及其对肺癌的影响.
- 确定基碳化合物受体 (AhR) 在这些过程中的作用.
主要方法:
- 由THP-1衍生的巨细胞暴露于1NP.
- 通过免疫光和油红O/尼罗河红色染色来评估AhR激活,核转位和脂质积累.
- 泡细胞条件介质用于治疗A549肺癌细胞.
- 使用AhR抑制来确定其作用.
主要成果:
- 1NP通过AhR激活诱导巨细胞中的泡细胞形成,导致脂质积累.
- 1NP诱导的泡细胞显示IL-6和TNF-α的表达增加和迁移增强.
- 来自1NP驱动的泡细胞的条件介质促进了A549细胞中的前瘤原始表型.
- 抑制AhR显著减弱了这些效应,突出了依赖AhR的近神经机制.
结论:
- 1NP诱导的泡细胞有助于支持瘤的肺微环境.
- 艾哈是关键的调解者,将环境中毒物暴露与免疫细胞重塑和癌症进展联系起来.
- 这项研究揭示了1NP和AhR在肺癌发生过程中的新作用.
关键词:
一-尼特罗皮尔烯 (Nitropyrene) 是一种1 - 尼特罗皮尔烯.啊啊啊啊啊啊啊啊啊啊啊啊啊啊啊啊啊啊啊啊环境毒理学环境毒理学肺癌是一种肺癌.帕拉克林效应的作用这就是THP-1的特点.瘤微环境是一个微环境.更多相关视频
相关概念视频
SN1 Reaction: Stereochemistry
10.2K
This lesson provides an in-depth discussion of the stereochemical outcomes in an SN1 reaction.
In the first step of an SN1 reaction, the bond between the electrophilic carbon and the leaving group ionizes to generate the carbocation intermediate. The second step of the mechanism is the nucleophilic attack.
In the formed carbocation, the positively charged carbon is sp2 hybridized with a trigonal planar geometry. As all the three substituents lie on the same plane, a plane of symmetry for the...
In the first step of an SN1 reaction, the bond between the electrophilic carbon and the leaving group ionizes to generate the carbocation intermediate. The second step of the mechanism is the nucleophilic attack.
In the formed carbocation, the positively charged carbon is sp2 hybridized with a trigonal planar geometry. As all the three substituents lie on the same plane, a plane of symmetry for the...
10.2K
SN1 Reaction: Kinetics
9.5K
In an SN2 reaction, the reaction rate depends on both the type of nucleophile and the substrate. A hindered tertiary alkyl halide is practically inert to the SN2 mechanism despite using a strong nucleophile.
However, Sir Christopher Ingold and Edward D. Hughes, who studied the kinetics of various nucleophilic substitution reactions, noticed that a tertiary alkyl halide does undergo a nucleophilic substitution reaction in the presence of a weak nucleophile. While studying the substitution...
However, Sir Christopher Ingold and Edward D. Hughes, who studied the kinetics of various nucleophilic substitution reactions, noticed that a tertiary alkyl halide does undergo a nucleophilic substitution reaction in the presence of a weak nucleophile. While studying the substitution...
9.5K
SN1 Reaction: Mechanism
14.1K
Kinetic studies of ionization of a tertiary halide in a protic solvent suggest that only the substrate participates in the rate-determining step (slow step). The nucleophile is involved only after the slowest step. The SN1 reaction takes place in a multiple-step mechanism.
Firstly, the haloalkane ionizes to generate a carbocation intermediate and a halide ion. This heterolytic cleavage is highly endothermic with large activation energy. The ionization of the substrate, facilitated by a...
Firstly, the haloalkane ionizes to generate a carbocation intermediate and a halide ion. This heterolytic cleavage is highly endothermic with large activation energy. The ionization of the substrate, facilitated by a...
14.1K
Acidity of 1-Alkynes
11.1K
The acidic strength of hydrocarbons follows the order: Alkynes > Alkenes > Alkanes. The strength of an acid is commonly expressed in units of pKa — the lower the pKa, the stronger the acid. Among the hydrocarbons, terminal alkynes have lower pKa values and are, therefore, more acidic. For example, the pKa values for ethane, ethene, and acetylene are 51, 44, and 25, respectively, as shown here.
11.1K
Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists
610
Neurokinin 1 (NK1) receptors are distributed across the GI tract, vagal afferents, and key CNS regions including the central vomiting center and chemoreceptor trigger zone (CTZ) Chemotherapy agents stimulate enterochromaffin cells in the gastrointestinal (GI) tract to release large amounts of substance P (SP). SP is a neuropeptide released by specific sensory nerves in response to many different stressors, including those in the GI mucosa affected by chemotherapy. SP binds and activates...
610
Predicting Products: SN1 vs. SN2
15.9K
Nucleophilic substitution reactions of alkyl halides can proceed via an SN1 or an SN2 mechanism. While in SN2 reactions, the nucleophile attacks the substrate simultaneously as the leaving group departs, in SN1 reactions, the substrate first dissociates to give the carbocation intermediate. Various factors such as the structure of the substrate, the strength of the nucleophile, and the nature of the solvent promote one mechanism over the other.
With increased substitution on the alkyl halide,...
With increased substitution on the alkyl halide,...
15.9K


