开发弹型Brassica napus单基因线路,以区分Plasmodiophora brassicae种族
Yan Zhang1, Hao Hu1, Jinghe Wang1
1Agriculture and Agri-Food Canada, Saskatoon Research and Development Centre, 107 Science Place, Saskatoon, Saskatchewan, S7N OX2, Canada.
概括
研究人员开发了六个菜单基因系 (SGLs),具有独特的俱乐部根抗性基因,以分类Plasmodiophora brassicae种族. 这种资源有助于监测病原体的多样性,并制定持久的球根抗药策略.
科学领域:
- 植物病理学 植物病理学
- 农作物科学 农作物科学
- 遗传学 遗传学 是一个
背景情况:
- 球根,由Plasmodiophora brassicae引起,对巴西菜作物构成重大威胁,特别是大麻 (Brassica napus).
- 俱乐部根的有效管理策略依赖于了解病原体多样性和部署耐药品种.
研究的目的:
- 开发弹型Brassica napus单基因系 (SGLs),每个系都含有独特的球根抗性 (CR) 基因.
- 用这些SGL来准确地分类加拿大西部的Plasmodiophora brassicae种族.
- 建立一个资源来监测病原体的演变,并指导培育策略,以获得持久的球根耐药性.
主要方法:
- 来自B. rapa和B. napus的CR基因通过交叉进入敏感DH16516系.
- 用标记器辅助选择和微胞培养来产生双状SGL的应用.
- 对于弹型生长,形态相比性和CR基因存在的SGLs的表征.
- 使用开发的SGLs将35个P. brassicae野外菌株分为24个种族.
主要成果:
- 成功开发了六个弹型B. napus SGL,每个都有一个独特的CR基因 (Rcr1,Rcr3,Rcr5,Rcr8,Rcr9或Rcr10).
- 这些SGL的生长和形态与敏感的复发性母体具有相似的弹型生长和形态.
- 对35个P. brassicae野外菌株的分析揭示了24个不同的种族,其激烈性等位基因频率在22.9%至57.1%之间.
- 没有一个单独的CR基因提供了普遍的耐药性,这表明需要基因堆叠才能获得全面的耐药性.
结论:
- 开发的弹型SGL为加拿大大麻作物背景的俱乐部根种族概况提供了一个精确的工具.
- 这些SGL对于监测病原体多样性和告知CR基因的部署至关重要.
- 这些发现强调了基因堆叠策略对于在甘油品种中实现持久的球根耐药性的重要性.
关键词:
布拉斯卡纳普斯 (Brassica napus) 是一种植物.俱乐部根抗性基因的基因这种病毒是Plasmodiophora brassicae.国家统一计划的标志物.不同的设置差异集.一个基因的线条.更多相关视频
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