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纳米孔向测序用于肺部感染的肺结核流行环境的诊断性能:一项前性观察性研究
Qijing Fan1, Jun Chen2, Ying Wang3
1Department of Gastroenterology, Ministry of Education Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Hubei Clinical Center and Key Laboratory of Intestinal and Colorectal Disease, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
概括
纳米孔向测序 (NTS) 在结核流行地区准确地检测出各种肺病原体,包括真菌菌,真菌和细菌. 这种方法提供了比复杂感染的常规检测更高的诊断产量.
科学领域:
- * 传染病诊断 传染病诊断 传染病诊断
- * 微生物学 微生物学
- * 基因组医学是一种基因组医学.
背景情况:
- *结核病 (TB) 流行地区的肺部感染复杂,通常由多种病原体引起.
- *纳米孔向测序 (NTS) 是一种有前途的技术,可以同时检测真菌菌,细菌和真菌.
- * 关于结核病流行群体中不同病原体类别的NTS表现的数据有限.
研究的目的:
- * 评估纳米孔向测序 (NTS) 在结核病流行环境中诊断肺部感染的性能.
- *将NTS与各种病原体类型的常规微生物学测试 (CMT) 进行比较,包括单个和多微生物感染.
主要方法:
- *前性研究,在一个专门的结核病医院招募305名怀疑肺部感染的成年人.
- *使用NTS和CMT分析的呼吸器样本 (n=312) .
- * 盲目的临床诊断作为参考标准.
主要成果:
- *在283例中,NTS在263例中发现了病原体,显著超过CMT (185例).
- * NTS对 * Mycobacterium tuberculosis * ,非结核菌和真菌表现出高度的敏感性和特异性.
- * NTS提供了12.2%的增量诊断产量,对于多微生物感染特别有价值.
结论:
- *NTS是一种敏感的,单项测试方法,用于检测结核流行地区的多种肺病原体.
- *NTS可以简化工作流程,并提高检测细节或共感染生物的速度.
- *临床相关性对于解释来自非无菌呼吸道样本的细菌NTS结果至关重要.
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