通过紫外线辐射使传染性病毒样本无活化,用于下游分析
Hugues Graf1, Sébastien Faure1, Maelle Mellano1
1Analytical Sciences Department, Sanofi - Campus Mérieux, 1541 Avenue Marcel Merieux, 69280, Marcy L'Etoile, France.
Journal of virological methods
|January 25, 2026
概括
紫外线C辐射有效地使腺病毒,病毒和狂犬病病毒等病毒失活. 这种方法保持了细菌内毒素测试的性能,允许安全地将样本从封闭区域转移.
科学领域:
- 病毒学 病毒学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 传染性样品的下游分析需要无活化,以便安全地从封装中转移.
- 紫外线-C (UV-C) 辐射是一种潜在的样本无活化方法.
- 保护样本的完整性,特别是细菌内毒素测试 (BET) 的性能至关重要.
研究的目的:
- 用UV-C辐射研究腺病毒,复原病毒和狂犬病病毒的无活化.
- 评估紫外线辐射对细菌内毒素测试 (BET) 性能的影响.
- 为了优化UV-C剂量,以有效的病毒失活和保存BET恢复.
主要方法:
- 病毒样本 (腺病毒,转病毒,狂犬病病毒) 含有内毒素的暴露在不同的UV-C剂量 (1-9J/cm2) 下.
- 辐射后病毒失活率和内毒素恢复率的分析.
- 对包括细胞培养基在内的样本矩阵对UV-C疗效的影响的研究.
主要成果:
- 一个UV-C剂量为5J/cm2 (2×2.5J/cm2) 实现了1mL体积中的病毒的完全无活化.
- 在测试的UV-C剂量和样本量中,内毒素恢复率保持在100%附近.
- 用细胞培养基稀释样品是必要的,以克服紫外线衰减并确保完全无活化.
结论:
- 优化的UV-C辐射条件确保有效的病毒失活,同时保持BET特异性.
- 在5J/cm2的UV-C处理适合在不影响内毒素检测的情况下使传染性病毒失活.
- 这种方法有助于安全地转移生物样本,如狂犬病疫苗,用于隔离区以外的测试.
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