相关实验视频

Updated: Jan 27, 2026

Aggravation of Myocardial Ischemia upon Particulate Matter Exposure in Atherosclerosis Animal Model

Aggravation of Myocardial Ischemia upon Particulate Matter Exposure in Atherosclerosis Animal Model

Published on: December 10, 2021

循环THBS1通过海绵化miR-18a-5p和与CTGF相互作用来加剧腹膜纤维化

Yanhong Guo¹, Silu Zhao¹, Xuewen Zhang¹

¹Department of Nephropathy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, People' s Republic of China.

The international journal of biochemistry & cell biology

|January 25, 2026

概括

循环RNAs (circRNAs) 参与腹纤维化,这是腹透析 (PD) 的并发症. 这项研究将circTHBS1确定为关键参与者,证明其作为治疗腹膜纤维化治疗的治疗点的潜力.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
腎臟醫學腎臟醫學

背景情况:

腹膜纤维化是长期腹膜透析 (PD) 的重要并发症.
已知循环RNAs (circRNAs) 参与器官纤维化.
围纤维化进展中circRNAs的特定作用以前尚不清楚.

研究的目的:

为了研究circRNAs在腹纤维化发展中的作用.
阐明围纤维化中circRNA参与的潜在分子机制.

主要方法:

来自PD患者的腹膜间皮质细胞中的circRNA表达概况.
在细胞系中使用基因沉默和过度表达的circTHBS1的功能研究.
在PD小鼠模型中的体内验证,通过AAV输送进行circTHBS1敲击.
对分子标记物和腹功能进行分析.

主要成果:

在PD患者中,CircTHBS1显著上调,并与纤维化标志物 (纤维素,α-SMA,CTGF) 相相关.
沉默circTHBS1在体外抑制了表皮细胞-介质细胞转换 (EMT) 并在体内减轻了腹膜纤维化.
循环THBS1将miR-18a-5p进行海绵,释放CTGF的抑制,并提高CTGF的稳定性.

结论:

在通过miR-18a-5p/CTGF轴促进腹纤维化方面,CircTHBS1起着至关重要的作用.
CircTHBS1代表了一种潜在的治疗点,用于治疗PD患者的腹膜纤维化.

关键词:

HMrSV5 5 的时间.圆圈THBS1 的时间表皮细胞-介质细胞过渡 (EMT)腹膜透析是指腹膜透析.腹膜纤维化腹膜纤维化

更多相关视频

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells

Published on: May 2, 2017

A Retrograde Implantation Approach for Peritoneal Dialysis Catheter Placement in Mice

A Retrograde Implantation Approach for Peritoneal Dialysis Catheter Placement in Mice

Published on: July 20, 2022

相关概念视频

Cystic Fibrosis: Pathogenesis

Cystic Fibrosis: Pathogenesis

Cystic fibrosis (CF), an autosomal recessive disorder, significantly affects the function of exocrine glands. This genetically inherited disease is characterized by the production of thick and sticky mucus, which can severely affect various organs and systems in the body.
CF is primarily caused by a genetic mutation in a chromosome 7 gene coding for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. The most common gene mutation leading to CF is the ΔF508 mutation,...

Peritoneal Dialysis II: Peritoneal Dialysis Systems and Complications

Peritoneal Dialysis II: Peritoneal Dialysis Systems and Complications

Peritoneal dialysis (PD) is a medical process that removes waste products and excess fluid from the body using the peritoneal membrane as a natural filter.Peritoneal Dialysis MethodsSeveral methods can be used for peritoneal dialysis, including Acute Intermittent Peritoneal Dialysis, Continuous Ambulatory Peritoneal Dialysis, and Automated Peritoneal Dialysis, also known as Continuous Cyclic Peritoneal Dialysis.Acute Intermittent Peritoneal Dialysis (AIPD) is used for patients with uremic...

Peritoneal Dialysis I: Introduction and Procedure

Peritoneal Dialysis I: Introduction and Procedure

Peritoneal dialysis (PD) is a procedure that facilitates the exchange of solutes, waste products, electrolytes, and excess fluid between the blood in the peritoneal capillaries and a dialysis solution introduced into the peritoneal cavity.Principles of Peritoneal Dialysis (PD)Diffusion: Waste products such as urea and electrolytes move from high concentrations in the blood to low concentrations in the dialysate across the peritoneal membrane. This mechanism is driven by the concentration...

Cystic Fibrosis: Management

Cystic Fibrosis: Management

Cystic fibrosis (CF) is an autosomal recessive disorder that predominantly affects individuals of Northern European descent, occurring at a rate of 1 in 3500. It is caused by a genetic mutation in a gene on chromosome 7, most commonly the ΔF508 mutation, that codes for the cystic fibrosis transmembrane conductance regulator (CFTR) protein. This results in thicker mucus secretions and obstruction pathologies in multiple organs, including the lungs and sinuses.
Sinus disease and chronic...

Peritoneal Dialysis III: Nursing Management

Peritoneal Dialysis III: Nursing Management

Peritoneal dialysis, or PD, utilizes the peritoneal membrane as a filter to eliminate excess fluid and waste products. Effective nursing management is essential for ensuring patient safety, preventing complications, and promoting optimal function of the peritoneal dialysis process.Assessment and MonitoringNurses must thoroughly assess the patient before, during, and after each dialysis session. Regular monitoring includes vital signs, daily weight, fluid intake and output, and laboratory values...

Predator-Prey Interactions

Predator-Prey Interactions

Predators consume prey for energy. Predators that acquire prey and prey that avoid predation both increase their chances of survival and reproduction (i.e., fitness). Routine predator-prey interactions elicit mutual adaptations that improve predator offenses, such as claws, teeth, and speed, as well as prey defenses, including crypsis, aposematism, and mimicry. Thus, predator-prey interactions resemble an evolutionary arms race.