从狗到人类的SFTSV直接传播:分子确认和风险评估
Zhifeng Li1, Shuyi Liang1, Hao Jiang2
1Department of Acute Infectious Diseases Control and Prevention, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu Province 210009, China.
概括
这项研究证实了从狗到人类的直接严重发烧与血小板缩小综合征病毒 (SFTSV) 传播. 狗便和唾液中的高病毒载荷表明,在特有地区的物主人存在重大风险.
科学领域:
- 兽医病毒学 兽医病毒学
- 动物传染病研究研究
- 分子流行病学分子流行病学
背景情况:
- 严重发烧与血小板缩小综合征病毒 (SFTSV) 是一种新兴的传播疾病,具有跨物种传播的潜力.
- 狗在SFTSV流行病学中的参与以及从狗传播到人类的可能性仍然不太清楚.
研究的目的:
- 为了调查严重发烧与血小板缩小综合征病毒 (SFTSV) 从狗传播到人类的可能性.
- 在自然感染的狗中描述病毒分泌模式.
主要方法:
- 来自受感染的狗及其主人的SFTSV菌株的全基因组测序.
- 定量逆转录聚合酶连锁反应 (qRT-PCR) 用于测量狗生物样本 (唾液,尿液,便) 中的病毒载荷.
主要成果:
- 在狗和主人身上发现了相同的SFTSV菌株,证实了遗传联系.
- 这只狗在血的便 (2.2 × 107副本/毫升) 和唾液 (2.3 × 105副本/毫升) 中表现出高的病毒载荷.
- 狗在整个疾病期间都观察到持续的病毒泄露,主人在狗患病14天后出现症状.
结论:
- 这项研究提供了第一份直接从狗到人类传播SFTSV的分子证据.
- 血的便和感染狗的唾液被确定为人类传播的关键来源.
- 加强犬类的SFTSV监测和物所有者的保护措施在特有地区至关重要.
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