促进皮肤伤口愈合的Cornau Cervi Degelatinatum提取物的分子机制:从转录组学和多模型验证的见解
Yuan Chang1, Yixiang Miao1, Xiutong Ge1
1School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, P.R. China.
Journal of ethnopharmacology
|January 25, 2026
概括
乙醇提取物Cornua Cervi Degelatinatum (CCD) 通过促进细胞生长和减少炎症,显著增强伤口愈合. 它丰富的氨基酸特征,特别是L-proline,支持其在组织修复方面的有效性.
科学领域:
- 药理学和传统医学的医学.
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 传统上,Cornua Cervi Degelatinatum (CCD) 是用于伤口愈合的.
- 目前尚不清楚CCD疗效背后的精确分子机制.
研究的目的:
- 评估CCD提取物 (CCE) 和CCD粉末的伤口愈合潜力.
- 研究涉及CCD介导伤口修复的分子途径.
主要方法:
- 使用全厚皮肤伤口模型与组织学和分子分析 (qRT-PCR,免疫组织化学).
- 在皮肤和内皮细胞上进行了体外测试 (CCK-8,流动细胞计,划伤,化疗).
- 进行RNA-seq进行转录基因分析和UPLC-QqQ-MS/MS进行氨基酸分析.
主要成果:
- CCE的乙醇提取物表现出卓越的伤口愈合活性,加速关闭并改善组织再生.
- 试验室研究显示,乙醇提取物增强了细胞增殖,迁移和化学反应.
- 在强效的乙醇提取物中发现了MAPK通路激活和特定氨基酸 (L-proline,hydroxyproline,L-arginine,L-serine) 的较高度.
结论:
- CCE,特别是乙醇提取物,通过MAPK路径调节有效促进伤口愈合.
- 乙醇提取物的独特氨基酸成分为其在伤口护理中的传统用途提供了科学依据.
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