对超热稳定古老的AIR合成酶的基质结合,域交换和耐热性的结构见解
Yu-Hung Chen1, Yen-Chieh Huang1, R Guru Raj Rao2
1Life Science Group, Scientific Research Division, National Synchrotron Radiation Research Center, Hsinchu, 300092, Taiwan.
International journal of biological macromolecules
|January 25, 2026
概括
这项研究揭示了来自古生物的高热稳定型5-aminoimidazole ribonucleotide synthetase (AIRS) 的结构,详细介绍了基质结合和独特的非域互换特征. 这些发现增强了对蛋白生物合成和热友蛋白结构的理解.
科学领域:
- 生物化学 生物化学
- 结构生物学 结构生物学
- 酶学 是一种酶学.
背景情况:
- 5-阿米诺伊米达核酸合成酶 (AIRS) 对于de novo纯素生物合成至关重要,催化了一个关键的ATP依赖的步骤.
- AIRS属于PurM超级家族,其特征是保存的ATPase域.
研究的目的:
- 阐明Pyrococcus abyssi (Pa) 和Pyrococcus horikoshii (Ph) 的高热稳定AIRS的基质结合部位和结构特征.
- 研究古老的AIRS中独特的非域互换特征及其对热稳定性的影响.
主要方法:
- 使用X射线晶体学来确定AIRS的结构与基质类似物,ATP类似物,产品和金属辅助因子复合.
- 进行了结构,突变和生物物理分析,以了解基质结合,催化和热稳定性.
主要成果:
- 晶体结构揭示了基质模拟物FGAR和ATP模拟物AMPPNP的结合位,稳定了活性位.
- 在古老的AIRS的N端域中发现了一种独特的非域互换特征,与细菌的正统学相对立.
- 热稳定性归因于增强的疏水性核心包装,加强的二元接口和缩短的表面循环.
结论:
- 该研究为理解AIRS中的基质结合,催化和域交换提供了结构基础.
- 这些发现有助于理解热友蛋白质的紧和坚固的结构以及纯素生物合成途径.
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