阿斯塔桑丁通过Sirtuin 1/AMPK/FOXO1通路保护视网膜的穆勒细胞免受高葡萄糖诱导的氧化应激
Teck Boon Tew1, Chang-Hao Yang2
1Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan; Department of Ophthalmology, National Taiwan University Hospital, Taipei 100, Taiwan; Department of Ophthalmology, National Taiwan University Hospital Hsinchu Branch, Hsinchu 300, Taiwan.
Experimental eye research
|January 25, 2026
概括
阿斯塔丁通过激活Müller细胞中的Sirtuin 1 (SIRT1) 来保护糖尿病视网膜病变. 这种抗氧化剂通过SIRT1/AMPK/FOXO1通路减少氧化应激和质激活.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 生物化学 生化学
背景情况:
- 糖尿病视网膜病变 (DR) 涉及氧化应激引起的视网膜微血管损伤.
- 穆勒质细胞是DR病变发生的关键参与者.
- 赛尔图因1 (SIRT1) 保护DR的线粒体损伤和亡.
研究的目的:
- 通过激活SIRT1.1,研究阿斯塔丁是否可以通过激活SIRT1.1,缓解高葡萄糖诱导的氧化应激在米勒细胞中.
- 探索阿斯塔克桑丁在DR中的保护作用背后的分子机制.
主要方法:
- 鼠视网膜的穆勒细胞 (rMC-1) 在高葡萄糖条件下用素治疗.
- 免疫组织化学和西部血栓检测评估了氧化应激和质激活 (GFAP).
- SIRT1抑制剂和siRNAs阐明了涉及SIRT1,AMPK和FOXO1.1的分子途径.
主要成果:
- 阿斯塔丁减少了活性氧物种 (ROS),恢复了谷氨,并保留了线粒体功能.
- 通过降低GFAP表达,阿斯丁抑制了质激活.
- 阿斯坦丁上调了AMPK,增加了NAD+水平,增强了SIRT1活性,并促进了FOXO1脱乙烯化.
结论:
- 阿斯塔丁在穆勒细胞中对高葡萄糖诱导的氧化应激产生保护作用.
- 保护机制涉及SIRT1/AMPK/FOXO1通路的激活.
- 阿斯塔丁显示出作为糖尿病视网膜病变治疗剂的潜力.
相关概念视频
01:28Nitric Oxide Signaling Pathway
6.2K
Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure...
6.2K
03:14Oxidation Numbers
42.3K
In redox reactions, the transfer of electrons occurs between reacting species. Electron transfer is described by a hypothetical number called the oxidation number (or oxidation state). It represents the effective charge of an atom or element, which is assigned using a set of rules.
42.3K
01:13Induced Pluripotent Stem Cells
27.3K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
27.3K
01:27C4 Pathway and CAM
49.0K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
49.0K
01:02Glucose Homeostasis: Regulation of Blood Glucose
4.0K
Carbohydrates consumed through foods are converted into glucose, a crucial energy source for the body. In the prandial state, high blood glucose levels stimulate the secretion of insulin from the pancreas. Insulin inhibits hepatic glucose production and stimulates glucose uptake and metabolism by muscle and adipose tissue. The excess glucose is converted into glycogen and stored in the liver and muscles.
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
During fasting, when blood glucose levels are low, the pancreas secretes glucagon. it...
4.0K
02:02Responses to Salt Stress
14.5K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
14.5K