在先前感染SARS-CoV-2的登革热患者免疫失调的试点蛋白质组分析
Elizabeth Cruz-Altamirano1, Miguel Angel Mayoral-Chávez1, Luis Román Ramírez-Palacios2,3
1Facultad de Medicina y Cirugía, Universidad Autonoma "Benito Juárez" de Oaxaca, Oaxaca, Mexico.
Frontiers in immunology
|January 26, 2026
概括
之前暴露于SARS-CoV-2可能会通过改变免疫反应而恶化登革热,可能会增加严重程度. 这项试点研究确定了免疫路径和生物标志物,表明SARS-CoV-2抗体与增强的登革热病毒复制之间存在联系.
科学领域:
- 免疫学和传染病.
- 蛋白质组学和生物标记物的发现.
- 病毒病原发生的病毒病原体.
背景情况:
- 登革热和SARS-CoV-2的同时传播引起了人们对抗体交叉反应的担忧,可能会加剧登革热的严重程度.
- 了解之前的SARS-CoV-2暴露对登革热病变的影响对于流行地区的公共卫生至关重要.
研究的目的:
- 探索之前的SARS-CoV-2暴露是否会调节登革热的病原性.
- 通过蛋白质基因分析和体外测试来确定涉及这种相互作用的潜在生物标志物和免疫通路.
主要方法:
- 跨截面观测试点研究涉及18名参与者,根据登革热和SARS-CoV-2IgG状态分为四个队列.
- 血清IL-6和C反应蛋白 (CRP) 水平的量化.
- 使用无标签的液体染色学-双重质谱法 (LC-MS/MS) 和体内抗体依赖增强 (ADE) 试验进行蛋白质组分析.
主要成果:
- 与登革热和抗SARS-CoV-2 IgG (NS1/SARS-DENV_IgG) 的队列相比,与只有登革热的组相比,表现出较轻微的登革热症状.
- 在抗SARS-CoV-2 IgG 组 (NS1/SARS_IgG) 的登革热患者中观察到 IL-6 和 CRP 水平升高.
- 蛋白质组分析揭示了参与补充/凝血和TGF-β信号通路的蛋白质的差异表达;体外ADE试验证实了抗SARS-CoV-2血清增强的DENV复制.
结论:
- 之前的SARS-CoV-2暴露可能通过抗体介导的免疫调节来增强登革热的严重程度,可能有利于Th2反应并增强病毒复制.
- 建议在共同流行地区对登革热风险分层进行新型候选生物标志物.
- 探索性和小样本大小需要在更大的纵向研究中进行验证.
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