在和物种中LILRB1和LILRB2的基因组和转录组学:多态,多样化和广泛的替代拼接
Natasja G de Groot1, Nanine de Groot1, Corrine M C Heijmans1
1Comparative Genetics and Refinement, Biomedical Primate Research Centre, Rijswijk, Netherlands.
Frontiers in immunology
|January 26, 2026
概括
我们在和身上特征了白细胞免疫球蛋白样受体B1 (LILRB1) 和LILRB2基因. 巨表现出广泛的LILRB1和LILRB2多样化,与人类和的保存谱不同.
科学领域:
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
- 灵长类动物遗传学
背景情况:
- 抑制性受体,如白细胞免疫球蛋白类受体B1 (LILRB1) 和LILRB2,对于调节免疫反应至关重要.
- 这些受体在各种免疫细胞上表达,作为免疫检查点抑制剂,可以与MHC I类分子相互作用.
- 了解LILRB1和LILRB2的遗传多样性和转录基因格局对于了解不同物种的免疫系统功能至关重要.
研究的目的:
- 在三种旧世界子物种中描述LILRB1和LILRB2的基因组和转录组概况: rhesus,长尾和Hamadryas.
- 在这些灵长类动物物种中调查LILRB1和LILRB2的等位基变异和家族遗传关系.
- 确定LILRB1和LILRB2转录组中的替代拼接模式及其对受体功能的潜在影响.
主要方法:
- 在PacBio平台上使用SMRT测序的全基因组测序被用来描述LILRB1和LILRB2.
- 生物信息分析被用来识别和描述等位基因,评估遗传多样性,并进行遗传学分析.
- 分析了转录基因数据,以识别替代拼接的异型,并描述拼接事件.
主要成果:
- 在 rhesus 和长尾中发现了大量的新型 LILRB1 (71) 和 LILRB2 (58) 的等位基因,只有一个以前发表的等位基因.
- 遗传学分析揭示了中LILRB1和LILRB2的广泛多样化,分别聚集成9个和12个不同的血统,与人类和哈马德里亚斯更保守的谱系形成鲜明对比.
- 在LILRB1和LILRB2转录组中,替代拼接的异型体占主导地位,主要影响干部区域和信号传导能力,在两种基因中都发现了特定的替代拼接事件.
结论:
- 与哈马德里亚斯和人类相比, rhesus 和长尾在 LILRB1 和 LILRB2 中表现出显著的遗传多样性.
- 替代拼接显著影响LILRB1和LILRB2转录组,这表明这些免疫受体具有复杂的调节机制.
- 这些发现为灵长类动物免疫检查点抑制剂的进化和功能变异提供了宝贵的见解.
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