Sir2 调节静止阶段酵母细胞中的选择性自
Ji-In Ryu1, Juhye Jung1, Jeong-Yoon Kim1
1Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Republic of Korea.
Microbial cell (Graz, Austria)
|January 26, 2026
概括
这项研究揭示了Sir2,一个组织素脱乙酶,在营养丰富的介质中抑制酵母静止阶段的选择性自. 删除SIR2激活了自,影响了器官细胞降解和细胞静止.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
背景情况:
- 自维持细胞平衡,特别是在营养限制期间.
- 在复杂介质中长期静止阶段对自的调节尚不清楚.
- Sir2是酵母中的NAD+依赖性基因素脱乙酶.
研究的目的:
- 研究Sir2在富含营养的复杂介质中静止阶段调节自的作用.
- 确定Sir2控制选择性自的机制.
- 了解Sir2在长期压力期间对细胞适应的贡献.
主要方法:
- 使用GFP-Atg8处理来测量自流量.
- 进行了转录组分析,以评估基因表达变化.
- 研究了关键的自成分 (如Atg32) 的蛋白质稳定和酸化.
- 在Saccharomyces cerevisiae中采用了基因删除策略 (SIR2删除).
主要成果:
- 在静止阶段,Sir2的删除导致持续的自活化.
- Sir2选择性地抑制了mitophagy,pexophagy和Cvt通路,而不是非选择性的自.
- Sir2稳定了Ume6,抑制了ATG8转录并限制了自.
- 删除SIR2增加了Atg32的稳定性和线粒,创造了一个ROS介导的正反循环.
结论:
- Sir2是一种新型抑制剂,可以在复杂介质中的酵母静止阶段抑制选择性自.
- Sir2调节核糖体生物发生和营养反应通路的静止状态.
- Sir2对有机细胞降解的控制对于在代谢压力下生存至关重要.
相关概念视频
Gas Chromatography: Types of Columns and Stationary Phases
2.3K
Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
2.3K
Yeast Signaling
17.2K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.2K
Autophagy
5.8K
Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
5.8K
pH Regulation in Cells
7.6K
pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
7.6K
Positive Regulator Molecules
136.1K
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
136.1K
Negative Regulator Molecules
38.5K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.5K


