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分裂酵母Whi5抑制了静止细胞中依赖MBF的转录
Celia Gálvez-Merchán1, Rafael López-San Segundo1, M Belén Suárez2,3
1Institute of Functional Biology and Genomics, CSIC, University of Salamanca, 37007 Salamanca, Spain.
iScience
|January 26, 2026
概括
在裂变酵母中,Whi5蛋白在静止期间进入核以抑制基因转录. Whi5促进MBF复合体和Clr6-I基因素脱乙酶之间的相互作用,抑制G1/S基因表达.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 细胞周期的进展依赖于精确调节基因转录.
- 静止,一种可逆的细胞循环停止,需要细胞循环促进转录因子的失活.
- 在芽的酵母和哺乳动物中,Whi5和视网母细胞瘤 (Rb) 蛋白抑制G1/S转录因子 (SBF和E2F).
研究的目的:
- 调查Whi5在核分裂酵母静止期间调节G1/S转录中的作用.
- 阐明Whi5调解转录抑制的分子机制.
主要方法:
- 窒素饥饿可以诱导静止.
- 定量质谱测量以确定蛋白质相互作用.
- 双分子光补充 (BiFC) 以可视化蛋白质复合体的形成.
- 基因表达分析以评估转录抑制.
主要成果:
- 在裂变酵母中,Whi5在饥饿时在核中积累.
- 在静止期间,Whi5对于抑制MBF依赖基因至关重要.
- Whi5在物理上与MBF复合体和Clr6-I基因组脱乙酶复合体相互作用.
- 对于MBF和Clr6-I.之间的关联,需要Whi5.
结论:
- 在裂变酵母中静止期间,Whi5充当G1/S转录的关键抑制剂.
- Whi5通过招募基因素脱乙酶活性到依赖MBF的基因促进剂来调节抑制.
- 这种机制突显了Whi5/Rb家族蛋白在细胞周期控制中的保留作用.
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