基于转录因子监管网络的前JSRV LTR促进者活动的调查
Xiaoyue Du1,2,3, Pei Zhang1,2,3, Xujie Duan1,2,3
1College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, China.
Frontiers in veterinary science
|January 26, 2026
概括
这项研究确定了GATA3和MEK/ERK通路是Jaagsiekte绵羊逆转录病毒 (exJSRV) 转录的关键调节者,对绵羊肺腺癌 (OPA) 的发展至关重要. 结果显示了GATA3的存在.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子生物学分子生物学
- 在瘤学瘤学.
背景情况:
- 绵羊肺腺癌 (OPA) 是绵羊和山羊的传染性呼吸道瘤,由Jaagsiekte绵羊逆转录病毒 (exJSRV) 引起.
- exJSRV长终端重复 (LTR) 控制病毒转录,但调节其活性的宿主因素基本上是未知的.
- 了解这些相互作用对于阐明OPA病原性至关重要.
研究的目的:
- 识别和验证调节 exJSRV LTR 活动的宿主转录因子和信号通路.
- 调查已识别的因素在OPA发展中的作用.
主要方法:
- 使用hTFtarget和AnimalTFDB进行 exJSRV LTR 相互作用转录因子的生物信息分析.
- 进行KEGG通路丰富分析,以确定相关的信号通路.
- 使用细胞培养 (报告测试,基因敲除/过度表达) 和动物模型 (裸体小鼠,C57BL/6小鼠) 的实验验证.
主要成果:
- 确定了与exJSRV LTR相互作用的53种潜在的转录因子,主要与MAPK/MEK/ERK通路有关.
- 激活MEK/ERK通路增强了exJSRV LTR转录;抑制减少了它.
- GATA3显著上调了exJSRV LTR活性和Env蛋白表达,在体内促进了瘤的形成.
- 在C57BL/6小鼠中, exJSRV LTR显示肺和肝组织的热带性.
结论:
- GATA3和MEK/ERK信号通路是exJSRV LTR活动的关键积极调节者.
- 这些发现为推动OPA的分子机制提供了新的见解.
- exJSRV LTR 显示了肺部和肝脏组织的潜在热带性.
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