通过优化稀释酸预处理,高固体酶化水解和高温发酵,高效的2G乙醇生产
Isabela S Ferreira1, Miguel A D Flores-Alarcón1, Inês C Roberto1
1Department of Biotechnology, Engineering College of Lorena, University of São Paulo (USP), Estrada Municipal do Campinho, N° 100, Campinho, Lorena, SP 12602-810, Brazil.
ACS omega
|January 26, 2026
概括
这项研究优化了2G乙醇生产的草预处理和水解. 高固体水解和酵母发酵实现了高效的乙醇产量,支持大规模生物燃料的可行性.
科学领域:
- 生物技术是生物技术.
- 可再生能源可再生能源是可再生能源.
- 化学工程是化学工程的重要组成部分.
背景情况:
- 越来越多的第二代 (2G) 乙醇需求需要提高经济可持续性.
- 优化预处理,高固体酶化水解和高温发酵是关键策略.
- 米草是生物燃料生产的丰富的纤维素生物质原料.
研究的目的:
- 用响应表面方法评估脱乙化米的稀释酸预处理.
- 为了优化高固体酶性水解和随后的2G乙醇的高温发酵.
- 评估大规模2G乙醇从米草生产的可行性.
主要方法:
- 通过响应表面方法优化米的稀释酸预处理.
- 将预处理扩展到一个80升反应堆.
- 在垂直球磨机 (VBM) 反应器中进行高固体酶化水解.
- 在43°C以*Kluyveromyces marxianus*进行水解酸的发酵,并补充营养成分.
主要成果:
- 在最佳的预处理条件下,产生了富含纤维素的固体 (58.2% w/w).
- 高固体水解实现了129g/L的可发酵糖,其纤维素转化率 (CCY) 为78.6%.
- 在水解剂+营养物质中的发酵产生了高的乙醇生产指标 (YP/S=0.46 g/g,QP=1.74 g/L/h, η=90%).
- VBM反应堆进一步增强了乙醇生产 (QP=3.04 g/L/h,37 g/L标位).
结论:
- 经过优化后续加工,有效地从米中产生富含葡萄糖的水解盐.
- 通过*Kluyveromyces marxianus**的高温发酵成功进行了.
- 该过程表明了大规模,经济可持续的2G乙醇生产的潜力.
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