相关实验视频
Updated: Jan 27, 2026
10:05
Ganglioside Extraction, Purification and Profiling
Published on: March 12, 2021
5.1K
从中提取,净化和识别总量沙素及其应用评估
Yuan Fang1, Wenjun Kan1, Yilizhati Yimamu1
1School of Laboratory Medicine (Pharmaceutical Sciences), Jilin Medical University, Jilin, China.
Frontiers in nutrition
|January 26, 2026
概括
这项研究优化了微波辅助提取 (MAE) 来从中提取素,达到34.61%的产量. 提取物显示出显著的抗癌和抗氧化活性,为质量控制和生物活性评估提供了一种新方法.
科学领域:
- 自然产品化学 自然产品化学
- 药理学是指药理学,即药理学是指药理学.
- 生物技术是生物技术.
背景情况:
- 含有具有潜在健康益处的生物活性化合物.
- 有效的提取方法对于分离和研究这些化合物至关重要.
- 氨酸是一种已知具有多种药理活动的化合物.
研究的目的:
- 开发和优化微波辅助提取 (MAE) 方法,从中提取素.
- 确定最佳的提取参数,以最大限度地提高SHM产量.
- 描述提取的化合物,并评估它们的抗癌和抗氧化活性.
主要方法:
- 使用了微波辅助提取 (MAE).
- 使用响应表面方法 (RSM) 来优化提取参数:固体-液体比,功率,持续时间和乙醇度.
- 超高性能液体色谱/四极点飞行时间质谱 (UPLC/Q-TOF-MS) 用于化合物识别.
- 进行了体外测试,以评估抗癌和抗氧化活性.
主要成果:
- 确定了最佳的提取参数:57.52%的乙醇,1:25 g/mL的固体/液体比率,20分钟的持续时间和369.75W的功率.
- 优化的MAE方法实现了最大SHM收益率34.61%.
- 在UPLC/Q-TOF-MS分析中,确定了22种化合物,包括基烯,腺素,固醇,纯氨酸和二烯.
- 该SHM提取物表现出显著的抗癌和抗氧化活性.
结论:
- MAE是一种有效和高效的方法,可以从中提取素.
- 优化的提取过程为SHM的质量控制和生物活性评估提供了有价值的方法.
- 这项研究是首次对中的素进行系统的研究,突出了它们的潜在治疗应用.
相关概念视频
01:12Total Voids in Concrete
477
Total voids in concrete encompass gel water volume, capillary pores, and entrapped air. Gel water (retained within the cement hydration products) and physically entrapped or adsorbed water are significant for the hydration process. For complete hydration, it's estimated that the space needed for the products of a cubic centimeter of cement doubles. Capillary pores constitute the unoccupied space within the hydrated cement paste, with their size largely influenced by the water-to-cement...
477
01:05Total Internal Reflection Fluorescence Microscopy
11.1K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.1K
00:53Extraction: Effects of pH
1.3K
Consider a neutral form of an amine, B, with a partition coefficient, K, in a liquid mixture containing organic and aqueous phases. The pH of the aqueous phase affects the charge on acidic and basic solutes, and the charged form is usually more soluble in the aqueous phase. Suppose the conjugate acid form of the amine is soluble only in the aqueous phase while the base form is soluble in both phases. Then the distribution coefficient, D, can be given as the ratio of amine concentration in the...
1.3K
01:24Static, Stagnation, Dynamic and Total Pressure
1.5K
The concept of static, stagnation, dynamic, and total pressure is fundamental in fluid dynamics, often explained using Bernoulli's equation:
1.5K
01:28Methods of Classification and Identification
1.1K
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
1.1K
01:18Detergent Purification of Membrane Proteins
6.4K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
6.4K