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病毒研究中的细胞系偏差:对病毒传播和生物解释的影响
Ji-Young V Kim1, Bradley S Pickering1,2
1National Centre for Foreign Animal Diseases, Canadian Food Inspection Agency, Winnipeg, MB, Canada.
Frontiers in cellular and infection microbiology
|January 26, 2026
概括
病毒学研究依赖于细胞系,但过度使用的细胞系会引入偏见. 利用具有相似基因表达特征的多样化,未充分利用的细胞系可以改善病毒研究.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 基因组学就是基因组学.
背景情况:
- 细胞系对于病毒的传播和在病毒学中的表征至关重要.
- 过度依赖少数历史细胞系 (例如,Vero,BHK-21,MDCK) 可以导致偏见和模糊的病毒生物学.
- 对6000多篇出版物的审查表明,不成比例地使用特定的细胞系.
研究的目的:
- 要突出病毒学研究中细胞系的有限选择带来的偏见.
- 倡导一种更具战略性和分子知情性的细胞系选择方法.
- 强调需要更广泛地使用生物多样性的细胞面板来研究病毒.
主要方法:
- 文学评论超过6000个病毒学出版物.
- 通常使用的基因表达分析与未充分利用的细胞系.
- 在不同细胞系类型中识别亲病毒过程基因.
主要成果:
- 在病毒学研究中,少量细胞系被不成比例地使用.
- 来自各种组织类型的未充分利用的细胞系显示出可比的亲病毒基因表达特征.
- 历史上的先行性和一般的宽容性有助于细胞系选择偏差.
结论:
- 对细胞系选择的数据驱动策略对于精确的病毒研究至关重要,特别是对于新兴和动物性病毒.
- 建议开发分子数据库,并在多种细胞系中确定病毒学相关的特征.
- 扩大细胞系的使用将提高可复制性,并对病毒病原发生产生更深入的见解.
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