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在更少的时间里找到更多:使用残留细胞学样本治疗胰腺癌的精准医学
Raquel Antón-Peñalver1, María Ortega2, Jose Francisco González-Muñoz2
1Departament de Patologia, Facultat de Medicina i Odontologia, Universitat de València, València, Spain.
概括
胰腺管腺癌 (PDAC) 的剩余液体基细胞学 (LBC) 适用于分子分析,即使在长期储存后也是如此. 保存这些样本可以确保患者能够获得有针对性的治疗方法.
科学领域:
- 在瘤学瘤学.
- 分子诊断学 分子诊断学
- 胃肠病学 胃肠病学
背景情况:
- 胰腺管腺癌 (PDAC) 是一种致命的恶性瘤,发病率越来越高.
- 准确的诊断依赖于病理确认,通常是通过内镜超声波引导细针吸收 (EUS-FNA).
- 剩余液体细胞学 (LBC) 样本用于PDAC分子测试的实用性尚未得到充分证实.
研究的目的:
- 评估用于PDAC分子分析的剩余LBC样本的实用性和储存条件.
- 为了确定剩余的LBC是否可以可靠地用于下一代测序 (NGS) 和其他分子测试.
- 评估储存时间对从LBC样本中核酸质量的影响.
主要方法:
- 从2020年至2024年通过EUS-FNA和LBC诊断的268名PDAC患者的回顾性分析.
- 储存了剩余的LBC样本,并根据可用性和质量进行了分子分析.
- 剩余LBC和细胞块之间的DNA和RNA质量和测试有效性的比较.
主要成果:
- 剩余的LBC是8名患者唯一可用的样本.
- 与细胞块相比,LBC中的DNA度更高,中位数绝对对对差 (MAPD) 较低.
- 液体细胞储存时间没有影响NGS有效性,结果与DNA和MSIRT-PCR细胞块相比.
- RNA分析产生了较少的有效研究,LBC和细胞块之间没有显著差异.
结论:
- 来自PDAC的剩余LBC样本可用于分子分析,保持核酸质量.
- 剩余的LBC可以作为分子测试的主要或唯一的样本.
- 保存剩余的LBC对于确保患者获得必要的向疗法至关重要.
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