在强酸性介质中进行细胞成像的新型pH响应式开启性ksanthone探针
Nirmal Chakraborty1, Pulakesh Pramanik1, Bappa Maiti1
1School of Applied & Interdisciplinary Sciences, Indian Association for the Cultivation of Science, Kolkata 700032, India.
The journal of physical chemistry. B
|January 26, 2026
概括
新的光探测器,Xpy和XpyF,使得在强酸性环境中可靠的细胞成像. 这些水溶性,生物相容的传感器为先进的生物成像应用提供启动式光和独特的pH依赖定位.
科学领域:
- 化学生物学 化学生物学
- 生物医学工程 生物医学工程
- 分析化学 分析化学
背景情况:
- 光探针对于细胞成像和监测pH依赖过程至关重要.
- 现有的探测器在强酸性环境 (pH < 4) 中经常失败,这对研究这些条件构成了挑战.
- 开发适用于极端酸性条件的水溶性,生物相容的探头是一个尚未满足的分析需求.
研究的目的:
- 设计和合成新的pH响应光探针,以在强酸性介质中可靠运行.
- 评估新探针的水溶性,细胞毒性和光性质.
- 研究探头在生物系统中的细胞吸收,定位和pH感应能力.
主要方法:
- 合成基于香的光探针 (Xpy和XpyF).
- 光物理性质的表征,包括光发射,量子产量和光寿命.
- 在各种pH条件下评估水溶性,细胞毒性和细胞成像.
- 使用皮尔森相关系数对探头定位的评估.
主要成果:
- 在酸性条件下,Xpy和XpyF表现出极好的水溶性,低细胞毒性和强烈的开启光.
- 探测器显示出明显的排放变化,从蓝色 (pH 3-7) 到绿色 (pH 1-2) 随着酸度的增加.
- 在酸性条件下观察到显著的光增强和寿命延长,这归因于质子化诱导的PET抑制.
- 细胞成像显示了高效的内部化和强大的核定位.
- XpyF显示了一个独特的pH依赖切换在定位从细胞质到细胞核.
结论:
- Xpy和XpyF是用于强酸性环境的有效核准光传感器.
- 这些探头在极端酸性条件下提供可靠的性能,用于监测pH依赖的生物过程.
- 探测器中的微妙结构修改可以导致生物成像中的独特功能结果,突出显示传感器设计的途径.
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