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在斑马鱼中可视化异生菌和代谢物,采用VUV激光吸附后离子化质谱成像

Anan Li¹, Shijie Lu¹, Xueqing Shang¹

¹MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, 510631, China.

Analytical and bioanalytical chemistry

|January 26, 2026

概括

一个新的真空紫外线-激光去吸附电离后质谱仪 (VUV-LDPI-MS) 系统提高了生物组织中的化学检测灵敏度. 这种先进的质谱成像 (MSI) 方法可以改善药物和代谢物的可视化,样本的准备工作最小.

科学领域:

分析化学分析化学
生物技术是生物技术.
化学成像技术化学成像技术

背景情况:

质谱成像 (MSI) 对于绘制各种领域的化学分布至关重要.
传统的MSI在检测灵敏度与空间分辨率方面面临局限性,原因是电离效率低.
电离后技术显著提高了电离效率,推进了质谱学.

研究的目的:

开发和验证一个新的真空紫外线-激光脱后离子化质谱仪 (VUV-LDPI-MS) 系统.
通过使用后电离,提高生物组织中化学物种的检测灵敏度.
评估系统在减少背景干扰和基板效应方面的性能.

主要方法:

低光子能量,长波长激光脱吸与真空紫外线 (VUV) 软电离的整合.
开发一个用于增强电离的VUV-LDPI-MS系统.
将VUV-LDPI-MS系统应用于斑马鱼组织部分,用于成像外源和内源化合物.

主要成果:

实现了 8.3 pg/spot 的甲蓝 (MB) 理论检测极限.
显著减少基质效应和样本背景干扰.
在斑马鱼组织中成功可视化了外源MB和内源代谢物,将分布与解剖学和光学图像相关联.

结论:

与传统的激光脱离/电离质谱法 (LDI-MS) 相比,VUV-LDPI-MSI提供了更高的电离效率和灵敏度.
该技术需要最小的样本准备,使其成为一个强大的替代方案.
VUV-LDPI-MSI显示了未来化学分析和生物样本成像方面的重大前景.

关键词:

在离子化后的激光脱吸过程中.质谱仪成像成像质谱仪成像真空的紫外线光是真空的紫外线光斑马鱼是一种斑马鱼.

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