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实时ATP成像揭示了分支新生代谢期间的代谢状态

Akiko Mii¹, Shinya Yamamoto¹, Masamichi Yamamoto¹

¹Department of Nephrology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

|January 26, 2026

概括

尿道在发育期间的分支依赖于糖解,尿道细胞对其抑制比介质细胞更敏感. 这突显了新陈代谢调节在生过程中的关键作用.

科学领域:

发展生物学发展生物学
代谢调节代谢调节代谢调节
脏生理学脏生理学

背景情况:

脏形成对脏发育至关重要,但基础代谢机制尚不清楚.
宫内环境干扰会影响脏供养,增加高血压和慢性脏疾病的风险.
在发育过程中,代谢状态和脏数量之间的联系机制需要进一步研究.

研究的目的:

研究能量代谢的作用,特别是细胞质氨酸5-三酸盐 (ATP) 水平在胚胎脏发育中的作用.
通过使用先进的成像技术,探索ATP在产过程中的时空动态.

主要方法:

使用转基因小鼠 (GO-ATeam2) 表达细胞质ATP-FRET生物传感器,用于胚胎脏的实时成像.
在单细胞分辨率下实时对甲基脏进行了体外成像.
评估了糖分抑制对尿道芽分支和细胞粘附的影响.

主要成果:

尿路芽细胞 (UB) 尖端细胞在分支神经发生过程中表现出明显较低的ATP水平,与UB茎和帽子介质细胞 (CM) 相比.
糖分离抑制抑制了UB分支,并降低了UB和CM细胞中的ATP水平,对UB细胞的影响更为明显.
减少糖解导致UB分支的减少,细胞粘附的改变 (N-cadherin表达) 和CM细胞的混乱,而氧化酸化抑制没有这种影响.

结论:

分支性新生高度依赖于糖解,特别是在早期发育期间的UB细胞中.
UB细胞对糖解抑制的敏感性比介质细胞更强.
代谢调节,特别是糖溶解,在分支产中起着重要作用.

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