新生儿小鼠口腔裂和肠道屏障完整性的功能评估使用使用室
Louis Berthet1, Camille Tardiveau1, Maryline Roy1
1INSERM U1149, Center of Research on Inflammation, Université Paris Cité.
Journal of visualized experiments : JoVE
|January 26, 2026
概括
最初的1000天对肠道健康至关重要. 本研究详细介绍了评估新生儿肠道透性的方法,有助于了解生命早期对长期健康的影响.
科学领域:
- 新生儿生理学 新生儿生理学
- 微生物组研究的研究.
- 胃肠道的健康状况
背景情况:
- 生命的前1000天对于肠道微生物组轴的发展至关重要.
- 早期肠道屏障功能会影响长期的健康和疾病易感性.
- 了解新生儿肠道透性是评估生命早期暴露的关键.
研究的目的:
- 描述一种将分子口服给新生小鼠的方法.
- 概述一种用于评估新生儿肠道透性的ex vivo技术.
- 建立一个评估早期肠道屏障功能的框架.
主要方法:
- 从生命第6天开始,使用专用针的小鼠口腔测量.
- 在新生儿结肠样本 (DOL-10到断奶) 上使用室内测试.
- 评估细胞间 (FITC-Dextran 4 kDa) 和细胞间 (HRP 44 kDa) 透性的情况.
主要成果:
- 在120分钟内对细胞和细胞间标记物通过的量化.
- 直接 (FITC) 和间接 (HRP) 测量探头流量,使用盘子阅读器.
- 确定了评估新生儿肠道通透性的方法.
结论:
- 描述的方法允许对新生儿肠道透性的可靠评估.
- 这项研究提供了研究早期暴露如何影响肠道完整性的工具.
- 这些发现有助于了解早期肠道微生物组对健康的长期影响.
相关概念视频
Assessing Body Temperature - Oral
1.6K
Here are the steps to accurately measure oral temperature using an electronic thermometer:
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
1.6K
Oral Cavity
3.1K
The oral cavity, or the mouth, is a complex structure in humans that plays a vital role in our day-to-day lives. Its role is not only in chewing and swallowing food; it also plays a role in speech and facial expressions.
Teeth: The teeth are the hardest structures in our bodies. Humans have two sets of teeth throughout their lifetime: deciduous (baby) teeth and permanent teeth. Each tooth consists of several parts: the crown (visible part), the root (embedded in the jaw), enamel (hard outer...
Teeth: The teeth are the hardest structures in our bodies. Humans have two sets of teeth throughout their lifetime: deciduous (baby) teeth and permanent teeth. Each tooth consists of several parts: the crown (visible part), the root (embedded in the jaw), enamel (hard outer...
3.1K
Physiological Barriers
5.2K
Physiological barriers are semi-permeable cellular structures restricting drug diffusion into intracellular compartments and tissues. There are six types of physiological barriers: blood endothelial, cell membrane, blood-brain, blood-cerebrospinal fluid (CSF), blood-placenta, and blood-testis barriers.
The blood endothelial barrier is the most porous of these. It allows all small ionized, un-ionized, and lipophilic molecules to pass through the endothelial lining into the interstitial space...
The blood endothelial barrier is the most porous of these. It allows all small ionized, un-ionized, and lipophilic molecules to pass through the endothelial lining into the interstitial space...
5.2K
Chambers of the Heart
10.3K
The human heart is a complex organ made up of four chambers: the right and left atria and the right and left ventricles. These internal chambers are separated by partitions known as the interatrial and interventricular septa. The exterior of the heart features a groove known as the coronary sulcus that demarcates the atria from the ventricles, while the anterior and posterior interventricular sulci distinguish between the two ventricles.
Deoxygenated blood from the body is received in the right...
Deoxygenated blood from the body is received in the right...
10.3K
Mucosal Barrier of the Stomach
2.2K
The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
2.2K
Surface Membrane Barriers
2.8K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
2.8K


