相关实验视频

Updated: Jan 28, 2026

Efficient Purification of Elastin-Like Polypeptides (ELPs) from E. coli Using an Organic Solvent-based Extraction and Precipitation Method
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Efficient Purification of Elastin-Like Polypeptides (ELPs) from E. coli Using an Organic Solvent-based Extraction and Precipitation Method

Published on: January 9, 2026

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从大肠杆菌中有效净化像拉斯的多 (ELP),使用基于有机溶剂的提取和沉方法

Joydeep Rakshit1, Feng Qu1, Saloni Darji1

  • 1Bindley Bioscience Center, Department of Chemistry, Purdue Institute for Cancer Research, Purdue University.

Journal of visualized experiments : JoVE
|January 26, 2026
PubMed
概括

一种基于有机溶剂的新方法快速从大肠杆菌中净化弹性样多 (ELP). 这种高效的工艺为各种生物医学应用提供高纯度的ELP,克服了传统净化技术的局限性.

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Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry11:12

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Gravimetry: Inorganic And Organic Precipitating Agents00:49

Gravimetry: Inorganic And Organic Precipitating Agents

In gravimetry, the precipitant is chosen carefully to obtain a pure solid that can be easily filtered. Common inorganic precipitants can be used to determine several cations and anions. In some cases, the formation of the same precipitate can be used to determine the cation and the anion. For example, the reaction of barium and chromate ions to give barium chromate is used to determine both barium and chromate. However, precipitates such as hydroxides, oxalates, and metal ammonium phosphates...
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Solvents01:12

Solvents

A solvent is a substance, most often a liquid, that can dissolve other substances. Here, the substance being dissolved is called a solute. When a solvent and a solute combine, they form a solution - a homogenous mixture of both the solvent and the solute. Water is a universal biological solvent. Its polar structure allows it to dissolve many other polar compounds. The ability of water to dissolve is governed by a balance between water molecules binding to each other and binding to the solute.
A...
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Precipitation Titration: Endpoint Detection Methods01:19

Precipitation Titration: Endpoint Detection Methods

In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
In the Volhard method, a standard excess of AgNO3 is first added to the...
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Precipitation and Co-precipitation01:17

Precipitation and Co-precipitation

Precipitation and coprecipitation methods can be used to separate a mixture of ions in a solution. In qualitative inorganic analysis, ions that form sparingly soluble precipitates with the same reagent are separated based on the differences in solubility products. For example, consider the separation of Cu(II) and Fe(II) ions by precipitation as insoluble sulfides. First, copper(II) sulfide is precipitated by the addition of acidic H2S, where the dissociation of H2S is suppressed. Adding H2S...
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Precipitation of Ions

Predicting Precipitation
The equation that describes the equilibrium between solid calcium carbonate and its solvated ions is:
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Precipitation Reactions

In a precipitation reaction, aqueous solutions of soluble salts react to give an insoluble ionic compound – the precipitate. The reaction occurs when oppositely charged ions in solution overcome their attraction for water and bind to each other, forming a precipitate that separates out from the solution. Since such reactions involve the exchange of ions between ionic compounds in aqueous solution, they are also referred to as double displacement, double replacement, exchange reactions, or...
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