猪心脏骤停模型使用植入式除器
Benjamin Stage Storm1, Knut Tore Lappegård2, Charlotte Björk Ingul3
1Nord University; UiT The Arctic University of Norway; Nordland Hospital Trust; benjamin.s.storm@nord.no.
Journal of visualized experiments : JoVE
|January 26, 2026
概括
这项研究引入了一种新的猪心脏骤停模型,使用可植入心脏转换器-除器 (ICD) 进行可靠的心室动 (VF) 诱导和复苏. 这种精细的方法提高了数据的准确性,并减少了在心脏骤停研究中的动物使用.
科学领域:
- 心血管研究研究心血管研究
- 翻译医学是一种翻译医学.
- 动物模型 动物模型
背景情况:
- 大型动物模型对于心脏骤停研究至关重要.
- 对于心室动 (VF) 诱导和除的传统方法面临诸如运动器件和不一致的成功率等挑战.
- 现有的技术往往导致动物资源的利用率很高.
研究的目的:
- 开发一种可重复的猪心脏骤停模型,以改善翻译研究.
- 建立一种可靠的方法来诱导心室动 (VF) 和使用可植入心脏转换器-除器 (ICD) 进行心脏转换.
- 为了在复苏期间实现精确的心内电心图 (ECG) 监测,遵守3Rs原则 (替换,减少,改进).
主要方法:
- 使用可植入式心脏转换器-除器 (ICD) 进行控制的诱导和心脏转换,使猪心室动 (VF).
- 在同一个动物中实施VF诱导和心脏转变的重复循环.
- 在整个复苏过程中,采用心内心电图 (ECG) 进行连续的,耐人工物节律监测.
主要成果:
- 在心室动 (VF) 诱导和心脏转变方面都取得了很高的成功率.
- 在11只猪中证明了自发循环的可靠恢复.
- 与表面心电图监测相比,显示出更好的工件耐受性.
结论:
- 基于ICD的猪模型为心脏骤停研究提供了一种标准化和可重复的方法.
- 这种方法增强了实验的一致性,并促进了病理生理学和复苏策略的可重复性调查.
- 该协议有效地减少了生理压力和动物数量,与伦理研究原则保持一致.
更多相关视频
07:18Author Spotlight: A Unique Mouse Model of Asphyxia-Induced Cardiac Arrest
Published on: April 14, 2023
2.3K
10:55A Piglet Perinatal Asphyxia Model to Study Cardiac Injury and Hemodynamics after Cardiac Arrest, Resuscitation, and the Return of Spontaneous Circulation
Published on: January 13, 2023
2.3K
相关概念视频
01:22Cardiac Output II: Effect of Stroke Volume on Cardiac Output
3.3K
Cardiac output (CO), the amount of blood the heart pumps per minute, is a parameter in cardiovascular physiology determined by stroke volume and heart rate. Stroke volume, the amount of blood pushed from one of the ventricles per heartbeat, is influenced by preload, afterload, and contractility.
Preload
Preload refers to the initial elongation of the cardiac myocytes before contraction and is related to the volume of blood filling the heart at the end of diastole, or end-diastolic volume. The...
Preload
Preload refers to the initial elongation of the cardiac myocytes before contraction and is related to the volume of blood filling the heart at the end of diastole, or end-diastolic volume. The...
3.3K
01:19Cardiac Output I:Effect of Heart Rate on Cardiac Output
2.5K
Cardiac Output
Cardiac output (CO) refers to the total amount of blood ejected by one of the ventricles in liters per minute (L/min). In a resting adult, CO ranges from 5 to 6 L/min, adjusting according to the body's metabolic requirements.
Effect of Heart Rate on Cardiac Output
Cardiac output adapts to metabolic demands during stress, physical activity, or illness. The autonomic nervous system regulates heart rate via the sinoatrial node. The parasympathetic nervous system decreases heart...
Cardiac output (CO) refers to the total amount of blood ejected by one of the ventricles in liters per minute (L/min). In a resting adult, CO ranges from 5 to 6 L/min, adjusting according to the body's metabolic requirements.
Effect of Heart Rate on Cardiac Output
Cardiac output adapts to metabolic demands during stress, physical activity, or illness. The autonomic nervous system regulates heart rate via the sinoatrial node. The parasympathetic nervous system decreases heart...
2.5K
01:29Cardiac Cycle
12.7K
The cardiac cycle refers to the sequence of events that occur in the heart from the beginning of one heartbeat to the next. It's characterized by alternating periods of contraction (systole) and relaxation (diastole) of the heart muscles.
During the cardiac cycle, blood flow through the heart is regulated entirely by changing pressure gradients. This sequence of events begins with the heart in a state of total relaxation, known as mid-to-late diastole, during which blood passively flows from...
During the cardiac cycle, blood flow through the heart is regulated entirely by changing pressure gradients. This sequence of events begins with the heart in a state of total relaxation, known as mid-to-late diastole, during which blood passively flows from...
12.7K
01:13The Cardiac Cycle
98.0K
The heart beats rhythmically in a sequence called the cardiac cycle—a rapid coordination of contraction (systole) and relaxation (diastole).
The Process
Electrical signals—sent from the sinoatrial (SA) node in the right atrial wall to the atrioventricular (AV) node between the right atrium and right ventricle—cause both atria to simultaneously contract. When the signal reaches the AV node, it pauses for approximately a tenth of a second, allowing the atria to contract and...
The Process
Electrical signals—sent from the sinoatrial (SA) node in the right atrial wall to the atrioventricular (AV) node between the right atrium and right ventricle—cause both atria to simultaneously contract. When the signal reaches the AV node, it pauses for approximately a tenth of a second, allowing the atria to contract and...
98.0K
01:17Exercise and Cardiac Output
1.9K
Regular physical activity is essential for maintaining cardiovascular health, with aerobic exercises being particularly effective. According to the American Heart Association, 150 minutes of moderate to intense aerobic exercise per week is recommended for a healthy heart. Aerobic activities may include brisk walking, running, bicycling, cross-country skiing, and swimming, ideally performed three to five times per week.
Sustained exercise increases the muscles' oxygen demand, which can be...
Sustained exercise increases the muscles' oxygen demand, which can be...
1.9K
01:26Imbalances in Cardiac Output
2.8K
The heart's primary function is to pump blood throughout the body, maintaining a balance between blood sent out (cardiac output) and blood returning (venous return). If this balance is disrupted, it can result in congestive heart failure (CHF), a severe condition where the heart becomes an inefficient pump, leading to inadequate blood circulation.
CHF can occur due to the failure of either side of the heart. Left-side failure leads to pulmonary congestion—the right side continues to send...
CHF can occur due to the failure of either side of the heart. Left-side failure leads to pulmonary congestion—the right side continues to send...
2.8K