昆虫病原性线虫的种植者基础的体内传播
Shawn A Steffan1, Sehrish Gulzar2, Camila Oliveira-Hofman2
1US Department of Agriculture, Agricultural Research Service, Vegetable Crops Research Unit; Department of Entomology, University of Wisconsin-Madison; steffan@entomology.wisc.edu.
Journal of visualized experiments : JoVE
|January 26, 2026
概括
农民现在可以使用两种新的,可靠的种植者系统生产自己的昆虫病原性线虫 (EPN) 用于昆虫害虫管理. 这些方法为传统的繁殖提供了可扩展和具有成本效益的替代方案,促进了农场的独立性和利能力.
科学领域:
- 农业昆虫学 农业昆虫学
- 生物控制剂 生物控制剂
- 尼马托德大规模传播
背景情况:
- 昆虫病原性线虫 (EPNs) 是对昆虫害虫的有效生物控制剂.
- 目前的in vivo EPN传播是高质量的,但劳动密集型和昂贵的.
- 现有的种植者系统由于依赖外部注射剂校准而缺乏可靠性.
研究的目的:
- 开发新的,以种植者为导向的系统,以实现可靠的,内部的EPN大众传播.
- 让农民能够独立生产自己的EPN生物杀虫剂.
- 为农业利益相关者提高EPN生产的可访问性和成本效益.
主要方法:
- 标准白色陷在体内EPN生产与以种植者为导向的聚烯胺凝基底法进行比较.
- 在聚合物涂层棉花二层内,Tenebrio molitor幼虫中的大规模传播的本地线虫物种 (Oscheius onirici,Heterorhabditis georgiana).
- 开发了一种系统的灌方法,用于从羊毛双层中收集线虫.
主要成果:
- 以种植者为导向的聚烯胺凝方法比标准的白色陷方法对Steinernema carpocapsae和Heterorhabditis bacteriophora产生了更多的线虫.
- 通过使用甲虫幼虫和棉花二层系统,成功地实现了本地EPN物种的大量繁殖.
- 收获方法有效地产生了适合野外应用的线虫泥.
结论:
- 开发的以种植者为导向的系统在技术上简单,可扩展和可靠,用于EPN生产.
- 这些方法使农民拥有可访问的工具来生产他们自己的生物杀虫剂.
- 广泛采用这些系统可以提高农场的独立性和利能力.
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