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亚细胞质谱学揭示了非对称地分裂 (青) 胚胎干细胞中的蛋白质组重塑

Bowen Shen¹, Leena R Pade¹, Fei Zhou¹

¹Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742.

Proceedings of the National Academy of Sciences of the United States of America

|January 26, 2026

概括

这项研究引入了先进的微探针毛细体电泳质谱法 (CE-MS),用于活体胚胎中的深层亚细胞蛋白质组. 该技术量化了数千种蛋白质,揭示了早期细胞命运规范至关重要的空间蛋白质组差异.

科学领域:

蛋白质组学是指蛋白质组学.
细胞生物学细胞生物学
发展生物学发展生物学

背景情况:

亚细胞蛋白质组学描述了特定细胞域内的蛋白质定位.
高分辨率成像补充了蛋白质组学,但在分析的蛋白质数量上有局限性.
从有限的生物样本中对亚细胞蛋白质组进行深度分析仍然是一个技术上的挑战.

研究的目的:

推进微探头毛细电泳质谱 (CE-MS) 结合被困离子移动性谱和数据独立获取 (diaPASEF) 进行深层亚细胞蛋白质组学.
量化来自异极的蛋白质不对称地分裂胚胎胚芽细胞在活的Xenopus laevis胚胎.
研究早期细胞命运规范期间蛋白质的空间分布和重塑.

主要方法:

开发和应用微探测器CE-MS与被困离子移动性光谱和diaPASEF.
在体内微试管采样从 *Xenopus laevis* 胚胎芽细胞体.
胚胎模式的扰乱,使用紫外线发泄来将空间分布与发育线索脱.

主要成果:

从~200 pg的HeLa消化物中量化了超过一千种蛋白质 (1,035) 的量化,具有高可重复性 (CV <15%).
在活胚胎中识别出808-1,022个来自背脊动物 (D1) 芽质体对立极的蛋白质.
在下属神经命运 (D1.1) 和表皮命运 (D1.2) 细胞中保留或重塑空间蛋白质分布的证明.
揭示了空间上不同的蛋白质分布,在前面和偏向,而不是修复,后来的细胞命运决定.

结论:

微探针CE-MS已被确立为完整胚胎中深度亚细胞蛋白质组学的强大工具.
在Xenopus laevis*早期的细胞命运规范过程中,存在空间上不同的蛋白质组.
这些蛋白质差异可能会影响,但不会预先确定,由诱导信号调节的后期细胞命运决定.

关键词:

这是Xenopus.布拉斯托梅尔分子是什么?质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量蛋白质组学蛋白质组学亚细胞亚细胞的情况.

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