间隙细菌生物膜是基于的结石的内在成分
William C Schmidt1,2,3,4, Ava Mousavi5, Jiahui Li6
1Department of Bioengineering, University of California Los Angeles, Los Angeles, CA 90095.
概括
氧化石是一种复杂的生物复合物,含有细菌生物膜. 这些生物膜,即使在非传染性石头中,通过促进矿物质核化,在结石形成中发挥着至关重要的作用.
科学领域:
- 生物矿物化 生物矿物化
- 微生物学 微生物学
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
背景情况:
- 氧化酸结石是最常见的结石类型.
- 目前的模型表明,岩石形成涉及无机晶石聚合.
- 细菌在非传染性结石形成中的作用尚不清楚.
研究的目的:
- 为了研究氧酸盐结石的内部结构.
- 确定细菌生物膜在岩石形成中的存在和作用.
- 提出结石病变的新模型.
主要方法:
- 电子显微镜和光显微镜被用来检查人类结石.
- 使用染色技术评估了细菌的存在和DNA.
- 分析了来自lithotripsy的石块碎片,以寻找生物膜结构.
主要成果:
- 结石表现出有机-无机的多晶生物复合体结构,与集成的细菌生物膜.
- 在石头内发现了生物膜,即使在临床上非传染病例和没有尿路感染的患者中也是如此.
- 生物膜附近的较小晶体尺寸表明核化位点增加,生物膜区域富含细菌DNA.
结论:
- 细菌是结石形成的内在因素,而不仅仅是感染性结石.
- 一个拟议的模型表明,尿液会影响细菌的平衡,导致细胞外DNA等核化模板的增加.
- 这一过程显著增加了氧化酸结石形成的概率.
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