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相关概念视频

Mass Spectrometers01:16

Mass Spectrometers

8.7K
This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
8.7K
IR Spectrometers01:25

IR Spectrometers

2.5K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
2.5K
Compact Bone01:27

Compact Bone

16.4K
Most bones contain compact and spongy osseous tissue, but their distribution and concentration vary based on the bone's overall function.
Compact bone, also called cortical bone, is the denser, stronger of the two types of bone tissue. It is found under the periosteum and in the diaphyses of long bones, where it provides support and protection. The microscopic structural unit of compact bone is called an osteon, or haversian system. Each osteon is composed of concentric rings of calcified...
16.4K
Compacting Factor test01:22

Compacting Factor test

576
The compacting factor test is a method used to assess the workability of concrete. It is  especially suitable for concrete mixes containing aggregates up to one and a half inches in size. This test involves specialized equipment consisting of two truncated cone-shaped hoppers and a cylinder, all with polished interior surfaces to minimize friction.
The procedure begins by placing concrete into the upper hopper without any compaction. Once filled, the bottom door of this hopper is opened,...
576
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

2.2K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
2.2K
UV–Vis Spectrometers01:14

UV–Vis Spectrometers

3.5K
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
3.5K

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相关实验视频

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Imaging Corrosion at the Metal-Paint Interface Using Time-of-Flight Secondary Ion Mass Spectrometry
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Imaging Corrosion at the Metal-Paint Interface Using Time-of-Flight Secondary Ion Mass Spectrometry

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一个新的紧型多反射飞行时间质谱仪.

Anatoly N Verenchikov1, Jason Wildgoose2, Sergey N Kirillov1

  • 1Mass Spectrometry Consulting Ltd, A5 JNA blv, Bar 85000, Montenegro.

Journal of the American Society for Mass Spectrometry
|January 26, 2026
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种新的液体染色学-质谱/质谱仪 (LC-MS/MS) 仪器,用于快速,准确地分析复杂的生物混合物,如脂质. 它平衡的速度,分辨率和动态范围为代谢学和蛋白质学提供了增强的性能.

关键词:
高分辨率的高分辨率解决方案脂质组的类型是什么多反射的飞行时间.

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Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
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Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry
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Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
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Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry
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科学领域:

  • 分析化学 分析化学
  • 生物化学 生物化学
  • 质谱测量质量谱测量

背景情况:

  • 质谱学对于分析复杂的生物混合物在代谢学,脂管学和蛋白质学等领域至关重要.
  • 对于这些应用,高速仪器具有ppm以下的质量精度和广泛的动态范围是必不可少的.

研究的目的:

  • 引入一种新的液体染色学质谱/质谱 (LC-MS/MS) 四极飞行时间质谱仪.
  • 为了突出其创新功能:一个新的碰撞电池,高动态范围探测器和紧的多反射直角飞行时间分析仪.

主要方法:

  • 开发和描述一个新的LC-MS/MS四极飞行时间质谱仪.
  • 评估仪器性能,包括分辨率,采集速度,动态范围和线性.
  • 在人血中对同位素标记脂质的分析,以证明能力.

主要成果:

  • 该仪器在100个频谱/秒的采集速度下达到10万个FWHM分辨率.
  • 在105动态范围内表现出极好的线性 (R=0.984).
  • 在人血中成功分析了同位素标记的脂质,显示出平衡的性能.

结论:

  • 新型LC-MS/MS仪器提供了速度,分辨率和动态范围的平衡组合.
  • 这种性能使其适合在代谢学,脂管学和蛋白质学中要求高的应用.
  • 该仪器代表了质谱学在复杂混合物分析方面的重大进步.