简化分散型固体相提取用于尿液中确定阿米特里林,可卡因和二甲基
Isabela Nucci Galetti1, Heloísa Peres Luz1, Bruna Espíndola da Silva1
1Pharmacy, Federal University of Santa Catarina, St. Delfino Conti S/N, Florianopolis, SC 88040-370, Brazil.
Journal of pharmacological and toxicological methods
|January 26, 2026
概括
开发了一种使用分散固相提取 (d-SPE) 和HPLC-DAD的新分析方法,用于检测可卡因,阿米和甲. 这种方法提供更低的检测极限和更快的分析时间,使其适用于紧急毒理学.
科学领域:
- 分析化学 分析化学
- 法医毒理学 法医毒理学
背景情况:
- 紧急毒理学需要快速准确的分析方法来检测药物.
- 现有的方法可能在检测极限,样本量或周转时间方面存在局限性.
研究的目的:
- 开发和验证一种分散型固相提取 (d-SPE) 方法与高性能液态染色学二极管阵列检测 (HPLC-DAD) 相结合.
- 评估该方法是否适合在紧急毒理学中快速检测可卡因,阿米特里普提林和二甲.
主要方法:
- 分散固态相提取 (d-SPE) 用于样品制备.
- 使用二极管阵列检测 (HPLC-DAD) 的高性能液体染色学,用于分析物分离和量化.
- 验证该方法的检测极限,精度,传递和稳定性.
主要成果:
- 达到可卡因的检测限值为100 ng/mL,而阿米三和二甲的检测限值为1300 ng/mL.
- 在不同度的分析物中,已证明精度低于15%.
- 方法显示没有转移,可卡因和阿米特里普提林的稳定性得到证实.
- 与具有较低检测极限和较小样本数量的其他技术相比,相对有利.
结论:
- d-SPE/HPLC-DAD方法是用于紧急毒理学分析的经过验证和有效的技术.
- 该方法为三个关键分析物提供了足够的灵敏度和14分钟的满意的总运行时间.
- 这种技术可以有效地补充现有的紧急毒理学工作流程.
相关概念视频
Filtration and Urine Formation
53.6K
The function of the kidneys is to filter, reabsorb, secrete, and excrete. Every day the kidneys filter nearly 180 liters of blood, initially removing water and solutes but ultimately returning nearly all filtrates into circulation with the help of osmoregulatory hormones. This process removes wastes and toxins but is also crucial to maintain water and electrolyte levels. Most of these functions are performed by the tiny but numerous nephrons contained within the kidneys.
53.6K
Phase Transitions: Sublimation and Deposition
20.0K
Some solids can transition directly into the gaseous state, bypassing the liquid state, via a process known as sublimation. At room temperature and standard pressure, a piece of dry ice (solid CO2) sublimes, appearing to gradually disappear without ever forming any liquid. Snow and ice sublimate at temperatures below the melting point of water, a slow process that may be accelerated by winds and the reduced atmospheric pressures at high altitudes. When solid iodine is warmed, the solid sublimes...
20.0K
Molecular and Ionic Solids
20.0K
Crystalline solids are divided into four types: molecular, ionic, metallic, and covalent network based on the type of constituent units and their interparticle interactions.
Molecular Solids
Molecular crystalline solids, such as ice, sucrose (table sugar), and iodine, are solids that are composed of neutral molecules as their constituent units. These molecules are held together by weak intermolecular forces such as London dispersion forces, dipole-dipole interactions, or hydrogen bonds, which...
Molecular Solids
Molecular crystalline solids, such as ice, sucrose (table sugar), and iodine, are solids that are composed of neutral molecules as their constituent units. These molecules are held together by weak intermolecular forces such as London dispersion forces, dipole-dipole interactions, or hydrogen bonds, which...
20.0K
Network Covalent Solids
16.1K
Network covalent solids contain a three-dimensional network of covalently bonded atoms as found in the crystal structures of nonmetals like diamond, graphite, silicon, and some covalent compounds, such as silicon dioxide (sand) and silicon carbide (carborundum, the abrasive on sandpaper). Many minerals have networks of covalent bonds.
To break or to melt a covalent network solid, covalent bonds must be broken. Because covalent bonds are relatively strong, covalent network solids are typically...
To break or to melt a covalent network solid, covalent bonds must be broken. Because covalent bonds are relatively strong, covalent network solids are typically...
16.1K
Phase Diagrams
50.0K
A phase diagram combines plots of pressure versus temperature for the liquid-gas, solid-liquid, and solid-gas phase-transition equilibria of a substance. These diagrams indicate the physical states that exist under specific conditions of pressure and temperature and also provide the pressure dependence of the phase-transition temperatures (melting points, sublimation points, boiling points). Regions or areas labeled solid, liquid, and gas represent single phases, while lines or curves represent...
50.0K
Urine Studies II: Urine Culture and Sensitivity Test
1.1K
A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
1.1K


![Solid-phase Synthesis of [4.4] Spirocyclic Oximes](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F58508.jpg&w=3840&q=50)