用JAR3D识别Rfam中的3D图案
James E Roll1, Craig L Zirbel2
1University of Findlay; rollj@findlay.edu.
概括
一个名为JAR3D的新工具可以在非蛋白质编码RNA中识别结构化的3D图案. 它分析了Rfam对齐中的循环,揭示了共同的结构模式,并帮助了功能性RNA研究.
科学领域:
- RNA结构生物学的RNA结构生物学
- 生物信息学是一种生物信息学.
- 计算生物学是一种计算生物学.
背景情况:
- 不编码蛋白质的RNA (ncRNA) 越来越多地被发现,需要了解它们的3D结构以实现功能.
- 经常出现的3D结构图案,如扭曲和GNRA循环,在各种ncRNA中发现.
- 识别这些图案有助于预测新发现的ncRNAs的功能.
研究的目的:
- 介绍JAR3D,一种用于识别RNA循环中已知的3D结构图案的工具.
- 为研究ncRNA结构和功能的研究人员提供这些识别.
- 分析RNA家族中已知的3D循环图案的流行情况.
主要方法:
- JAR3D是为了将RNA循环序列映射到已知的3D结构图案而开发的.
- 该工具被扩展到包括三向和四向交叉点的图案.
- 应用了JAR3D对4,166个Rfam种子对齐 (Rfam 15.0),结果可以在网上获得.
主要成果:
- 在大量的Rfam对齐中,JAR3D成功地确定了匹配的3D循环图案.
- 使用外部3DRNA结构的验证证实了JAR3D的准确性.
- 一个新的网页允许在所有Rfam家族中搜索特定的图案发生.
结论:
- JAR3D提供了一种可访问的方法,用于在序列对齐中识别已知的3DRNA循环图案.
- 该工具有助于研究RNA家族的基因分布和潜在的功能影响.
- 与发针和多螺旋连接相比,内部环对已知的图案的匹配率更高.
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