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肠道细胞中1,3和1,4结合的多糖的吸收依赖于克拉特林/动胺1/Rab5依赖性内细胞分裂

Wenfeng Liao¹, Dianxiu Cao¹, Ying Wang¹

¹Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.

Nature communications

|January 26, 2026

概括

多糖可以穿越肠道屏障,进入循环和组织. 这种吸收涉及特定的细胞机械,如克拉重链 (CLTC) 和膜受体,为口服药物开发铺平了道路.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
药理学药理学是指药理学的学科.

背景情况:

多糖通常被认为过于大和水友,无法穿透肠道粘膜进入循环.
了解多糖的吸收对于开发口服药物输送系统至关重要.

研究的目的:

为了研究肠道多糖体吸收的机制.
为了确定参与多糖体吸收的细胞组件和受体.

主要方法:

标记的多糖类 (β-1,3-葡萄糖,α-1,4-葡萄糖) 的口服给老鼠和小鼠.
使用基因敲除策略和抑制剂,准重链克拉特林 (CLTC),Rab5和dynamin1.
使用膜受体分析,包括骨形态遗传蛋白受体IA型 (BMPRIA),Dectin-1和表皮生长因子受体 (EGFR).

主要成果:

在口服后,在血和肝脏中检测到不同的链接和充电的多糖.
在肠道细胞中,多糖的吸收被证明需要克拉重链 (CLTC),Rab5和dynamin1.
在缺乏CLTC,RAB5A或DNM1的小鼠中,吸收显著降低,特定的膜受体被确定为内部化至关重要的.

结论:

这项研究表明,多糖类可以通过依赖于克拉的内分细胞分裂途径通过肠道粘膜被吸收.
特定的膜受体 (BMPRIA,Dectin-1,EGFR) 在某些多糖的内化中起着至关重要的作用.
这些发现为多糖体吸收机制提供了新的见解,并支持开发基于口服多糖体的治疗方法.

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