大麻烟雾提取物破坏了热囊细胞分化,并导致超出单独的Δ9-THC影响的线粒体功能障碍
Cristina Monaco1, Mahek Minhas1, Tina Podinic1
1Department of Pediatrics, Faculty of Health Sciences, McMaster University, 1280 Main St. W., Hamilton, ON, L8S 4K1, Canada.
Scientific reports
|January 26, 2026
概括
大麻烟雾提取物 (CaSE) 损害了胎盘细胞的发育和功能,与分离的THC不同. 这突出了怀孕期间吸入大麻的独特风险,影响胎儿发育.
科学领域:
- 生殖生物学 生殖生物学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 吸烟是怀孕期间大麻消费的主要方式.
- 以前的研究集中在单独的大麻化合物上,忽视了暴露于烟雾的复杂影响.
- 大麻烟对人类胎盘发育的影响尚不清楚.
研究的目的:
- 为了研究大麻烟雾提取物 (CaSE) 与 Δ9-THC 对人类热囊细胞分化的影响.
- 为了比较细胞和分子对整个大麻烟雾暴露的反应,与孤立的 Δ9-THC 相比.
- 评估CaSE对胎盘细胞功能,氧化应激和生物能学的影响.
主要方法:
- 使用了人体热囊细胞分化的体外模型.
- 暴露于大麻烟雾提取物 (CaSE) 和Δ9-四大麻醇 (Δ9-THC) 的细胞.
- 测量CYP1A1表达,hCG蛋白水平,syncytin-1基因表达,活性氧物种,线粒体呼吸和抗氧化基因变化.
主要成果:
- CaSE,但不是Δ9-THC,诱导了CYP1A1的表达,表明燃烧副产品的暴露.
- CaSE降低了hCG和syncytin-1的水平,这表明syncytialization受损.
- 导致CASE导致了剂量依赖的线粒体功能障碍和改变了氧化应激反应.
- CB1R对抗性减轻了 Δ9-THC 影响,但没有对差异化的 CaSE 影响.
结论:
- 与孤立的Δ9-THC相比,暴露于大麻烟雾对胎盘发育有明显和显著的不良影响.
- CaSE 破坏了胎盘的关键功能,包括细胞分化和能量代谢.
- 评估怀孕期间大麻使用的临床前模型必须考虑到暴露于烟雾的影响.
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