非热气压等离子体促进了Neurospora crassa中的细胞酶的产生
Nan-Nan Yu1,2, Wirinthip Ketya1, Kirubel Amsalu3
1Plasma Bioscience Research Center, Department of Plasma-Bio Display, Kwangwoon University, Seoul, 01897, Korea.
Bioresources and bioprocessing
|January 26, 2026
概括
使用非热气压等离子体可以增强微生物酶的产生. 这项研究表明,微表面介电屏障放电 (MS-DBD) 血通过改变细胞膜和信号通路来增加Neurospora crassa中的细胞酶生产.
科学领域:
- 生物技术是生物技术.
- 微生物生理学 微生物生理学
背景情况:
- 丝状真菌是工业酶生产的关键,但目前的方法需要提高产量和成本.
- 非热大气压等离子体 (NTP) 是一种用于刺激微生物过程的新技术.
研究的目的:
- 评估微表面介电屏障放电 (MS-DBD) 血,以增强Neurospora crassa中的细胞分解酶的产生.
- 为了研究血诱导酶增强的潜在细胞机制.
主要方法:
- 用MS-DBD等离子体治疗神经.
- 测量细胞外细胞酶活性和细胞酶基因转录.
- 分析真菌膜变化,细胞内 (Ca2+) 和氧化 (NO) 水平.
- 对Ca2+通道抑制剂和氧化通路的研究.
主要成果:
- MS-DBD等离子体治疗显著增加了细胞外细胞酶活性 (高达10.41%).
- 细胞酶基因的转录升高,在120s治疗时达到峰值诱导.
- 血治疗改变了真菌的细胞膜,增加了细胞内Ca2+和NO,并激活了Ca2+的流入.
- Ca2+通道抑制通过降低NO的调节来降低细胞酶的产生.
结论:
- MS-DBD血是一种有希望的,安全的工具,可以增强真菌酶的生产.
- 血诱导的酶增强包括膜脱极化,Ca2+信号传递和NO通路.
- 等离子技术可以应用于生物反应器,用于工业规模的酶生产.
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