通过脱化进行无氧高细胞密度培养的必要条件:技术和生理学的观点
Marte Mølsæter Maråk1, Ingrid Malien Duister1, Lars Reier Bakken1
1Faculty of Biotechnology, Chemistry and Food Science, Norwegian University for Life Sciences, Ås, Norway.
Microbial cell factories
|January 26, 2026
概括
使用脱利用单细胞蛋白 (SCP) 的无氧高细胞密度培养 (HCDC) 是可行的,实现高生物质和蛋白质含量. 然而,CO2-pH滞后,混合问题和酸盐毒性限制了生产率.
科学领域:
- 生物技术是生物技术.
- 微生物工程 微生物工程
- 可持续的粮食生产可持续的粮食生产
背景情况:
- 单细胞蛋白 (SCP) 为食品和料提供了传统农业的可持续替代品.
- 高细胞密度培养 (HCDC) 对于高效的SCP生产至关重要,但在有氧系统中面临氧气限制.
- 提出了一种使用酸盐作为电子受体和脱驱动化的厌氧策略,以克服氧气限制.
研究的目的:
- 评估无氧HCDC的可行性并确定使用SCP生产的脱化方法的局限性.
- 在这个系统中,研究在高细胞密度下限制生长速度的因素.
- 为优化无氧SCP生产提供见解.
主要方法:
- 在3L料批生物反应器中使用了Paracoccus denitrificans和Paracoccus pantotrophus.
- 采用pH-stat系统,利用脱驱动的化进行基板养.
- 进行了in vivo和in silico实验,以分析生长约束.
- 进行小批量生物测试以确定抑制性化合物.
主要成果:
- 达到高达60g/L干重的生物质度和75%蛋白质含量.
- 与低密度培养相比,在高细胞密度下观察到明显较低的生长率.
- 确定了主要的CO2-pH滞后,混合限制和酸盐毒性作为相互作用的约束因素.
- 证明降低pH设定值以减轻CO2-pH滞后会加剧酸盐毒性.
结论:
- 通过脱的无氧HCDC是一种生产高质量的SCP生物质的可行方法.
- 由于混合不良和酸盐毒性而加剧的CO2-pH滞后是竞争性生产率的主要障碍.
- 未来的研究应该集中在反应堆工程上,以改善混合和气体去除,以及为增强应力耐受性开发菌株.
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