一个3D模型来评估异构瘤微环境中的细胞化学反应
Daniel B Rodrigues1,2, Daniela Cruz-Moreira1,2, Luca Gasperini1,2
13B's Research Group, I3Bs - Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Rua Ave 1, Edifício 1 (Sede), 4805-694, Barco, Guimarães, Portugal. rpirraco@i3bs.uminho.pt.
Lab on a chip
|January 27, 2026
概括
研究人员开发了一种新的3D培养系统,用于研究瘤微环境中的细胞相互作用. 该系统观察到肌细胞向黑色素瘤细胞的定向迁移,有助于癌症入侵研究.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 生物材料科学 生物材料科学
背景情况:
- 瘤微环境 (TME) 是复杂而异质的,影响瘤的进展和入侵.
- 了解TME内的细胞相互作用对于开发新的癌症疗法至关重要.
- 当前的模型往往无法完全回顾体内TME动态.
研究的目的:
- 为了介绍一个新的,可定制的4井3D培养室,用于研究化学反应.
- 在3D原基质中研究不同类型的树皮细胞向黑色素瘤细胞的迁移.
- 评估该系统在模拟癌细胞招募和入侵方面的潜力.
主要方法:
- 开发一个使用I型原蛋白水凝作为基质的4井3D培养室.
- 在水凝中,基于分子重量的差分分子扩散是特征.
- 共同培养VMM15黑色素瘤细胞 (中心井) 与人类脂肪干细胞 (hASC),人类皮肤微血管内皮细胞 (hDMEC) 和人类皮肤纤维细胞 (hDFbs) (外部井).
主要成果:
- 流体细胞 (hASC和hDFbs) 在3D原基质内向VMM15黑色素瘤细胞呈现方向迁移.
- 与hASCs相比,人类皮肤纤维细胞 (hDFbs) 的迁移区域明显更大.
- 该系统证明了差异性的分子扩散,与分子重量相关.
结论:
- 这种新的3D培养系统有效地模拟了TME中的化学反应和细胞招募.
- 这种平台可以用来研究状细胞支持对黑色素瘤细胞入侵的影响.
- 使用该系统的进一步研究可以阐明瘤进展的机制,并为治疗策略提供信息.
相关概念视频
The Tumor Microenvironment
7.8K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
7.8K
Chemotaxis and Direction of Cell Migration
4.6K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
4.6K
Chemotaxis in E. coli
783
Chemotaxis in Escherichia coli is a sensory-driven motility mechanism that enables bacteria to navigate chemical gradients, moving toward beneficial environments while avoiding harmful conditions. This process relies on a signal transduction system integrating external chemical cues with flagellar motor control.Chemoreceptors and Signal DetectionE. coli detects chemical gradients through methyl-accepting chemotaxis proteins (MCPs), which are membrane-bound chemoreceptors that sense attractants...
783
Self-Evaluation Maintenance Model
290
The Self-Evaluation Maintenance (SEM) model offers a psychological framework to understand how individuals’ self-esteem is influenced by the achievements of others, particularly those with whom they share close personal bonds. The SEM model operates when personal rather than social identity guides individuals. Central to this model is the notion that individuals have an inherent desire to preserve a favorable self-image, which is continuously shaped by interpersonal comparisons and...
290
Cancer Stem Cells and Tumor Maintenance
6.0K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
6.0K
Tumor Immunotherapy
1.9K
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
1.9K


