电解质门有机电化学晶体管用于在急性损伤中动态检测HMGB1
Bo Wang1, You Kuai2, Zhihui Wang3
1Laboratory of Molecular Materials and Devices, College of Smart Materials and Future Energy, Fudan University, Shanghai 200438, China.
ACS applied materials & interfaces
|January 27, 2026
概括
这项研究引入了一种新的生物传感器,用于快速检测高流动性组盒1 (HMGB1),这是损伤的关键指标. 这项技术有望更早地诊断和监测急性损伤 (AKI),以预防慢性病 (CKD).
科学领域:
- 生物医学工程 生物医学工程
- 分子诊断学 分子诊断学
- 腎病理生理學 腎病理生理學
背景情况:
- 早期发现急性损伤 (AKI) 对于预防慢性病 (CKD) 的进展至关重要.
- 像血清肌素这样的传统生物标志物对早期AKI警告的敏感性有限.
- 高流动性组第1框 (HMGB1) 是损伤和修复的有希望的动态指标.
研究的目的:
- 开发一个快速和选择性的生物传感平台来检测HMGB1.
- 使用有机电化学晶体管 (OECT) 进行敏感信号传导.
- 建立一个用于早期诊断和损伤预后的工具.
主要方法:
- 制造了一种电解质开关的OECT生物传感器,其功能与用于识别HMGB1的DNA吸收体相结合.
- 使用OECT主动通道中的混合离子电子传输来进行信号传导.
- 采用脉冲驱动测量以提高灵敏度和快速获取信号.
主要成果:
- 该OECT生物传感器在广泛范围内 (5 pM到50 nM) 展示了选择性和快速检测HMGB1.
- 传感器在各种生理条件下可靠运行,包括血清.
- 缺血性AKI小鼠模型中的电反应与AKI到CKD过渡期间的HMGB1波动有定量相关.
结论:
- 为快速监测HMGB1建立了一个基于OECT的强大的生物传感策略.
- 这个平台为早期诊断和损伤的预后评估提供了一个有前途的工具.
- 开发的生物传感器可促进持续监测,有助于预防CKD的进展.
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