Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Phase Diagrams02:39

Phase Diagrams

50.0K
A phase diagram combines plots of pressure versus temperature for the liquid-gas, solid-liquid, and solid-gas phase-transition equilibria of a substance. These diagrams indicate the physical states that exist under specific conditions of pressure and temperature and also provide the pressure dependence of the phase-transition temperatures (melting points, sublimation points, boiling points). Regions or areas labeled solid, liquid, and gas represent single phases, while lines or curves represent...
50.0K
Phase Diagram01:19

Phase Diagram

7.0K
The phase of a given substance depends on the pressure and temperature. Thus, plots of pressure versus temperature showing the phase in each region provide considerable insights into the thermal properties of substances. Such plots are known as phase diagrams. For instance, in the phase diagram for water (Figure 1), the solid curve boundaries between the phases indicate phase transitions (i.e., temperatures and pressures at which the phases coexist).
7.0K
X-ray Crystallography02:18

X-ray Crystallography

26.1K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
26.1K
Rational Emotive Behavior Therapy01:24

Rational Emotive Behavior Therapy

402
Cognitive-behavioral therapies (CBTs) are grounded in the belief that our thoughts profoundly influence our emotions and actions. Advocates of CBT emphasize three core assumptions: first, that cognitions are identifiable and measurable; second, that they are central to psychological functioning; and third, that irrational or maladaptive beliefs can be replaced with rational and adaptive ones. This transformative approach to therapy has paved the way for specific models such as Albert...
402
Energy Diagrams - II01:10

Energy Diagrams - II

14.0K
Energy diagrams are important to understand the dynamics of a system. The topology of an energy diagram helps illustrate the equilibrium points of the system.
The point in the energy diagram at which the system’s potential energy is the lowest is known as the local minima. The system tends to stay in this position indefinitely unless acted upon by a net force. The slope of the potential energy diagram at the local minima is zero, indicating that zero net force is acting on the system. The...
14.0K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

7.0K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
7.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Correction to "Monooxygenase Activity of Indoleamine 2,3-Dioxygenase".

Journal of the American Chemical Society·2026
Same author

Drop-on-fixed-target reaction initiation approach for serial and time-resolved crystallography.

IUCrJ·2026
Same author

Cryo-EM reveals multiple mechanisms of ribosome inhibition by doxycycline.

Nature communications·2026
Same author

Mapping the effects of specific radiation damage and solvent radiolysis in buffers and crystals with online UV-Vis absorption spectroscopy.

Acta crystallographica. Section D, Structural biology·2026
Same author

Compact tape-driven sample delivery system for serial femtosecond crystallography.

Journal of applied crystallography·2026
Same author

Structural basis of the promiscuity of the unusual Fe(II) and 2-oxoglutarate dependent human aspartate/asparagine-β-hydroxylase.

Nature communications·2026

相关实验视频

Updated: Jan 28, 2026

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
09:09

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Published on: September 20, 2016

11.9K

自动化微批次油下相位图,以合理化序列结晶学样本制备过程.

Jack Stubbs1, Courtney J Tremlett2, Abigail Waitman1

  • 1School of Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom.

IUCrJ
|January 27, 2026
PubMed
概括

这项研究引入了一种自动化微批次油下选方法,用于快速蛋白质结晶选,大大减少了对串行结晶学样本的需求. 该技术优化了微晶的生产,用于先进的衍射实验.

关键词:
微批量-在油下-微批量微晶体是一种微晶体.优化的优化优化优化.阶段图 阶段图 阶段图样品的准备 样品的准备播种播种播种 播种播种连续晶体学是指连续的晶体学.

更多相关视频

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
09:52

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

Published on: February 4, 2021

2.7K
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
07:55

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

Published on: July 6, 2019

13.8K

相关实验视频

Last Updated: Jan 28, 2026

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
09:09

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

Published on: September 20, 2016

11.9K
Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
09:52

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

Published on: February 4, 2021

2.7K
An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
07:55

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering

Published on: July 6, 2019

13.8K

科学领域:

  • 结构生物学 结构生物学
  • 生物化学 生物化学
  • 晶体学 晶体学是指结晶学.

背景情况:

  • 序列结晶学需要高密度的微晶悬浮物.
  • 对微晶生产的样本优化是一个瓶.
  • 传统的相位图映射消耗过多的蛋白质样本.

研究的目的:

  • 开发一种快速,低体积的方法来绘制蛋白质结晶阶段边界.
  • 为了优化微晶密度,大小和晶格顺序,用于串行晶体学.
  • 为已知的和新的蛋白质标提供可复制的工作流.

主要方法:

  • 自动化微批次油下结晶.
  • 诊断采样策略不同蛋白质与沉物比率.
  • 多种沉物的相关变化和自动化种子库定位.

主要成果:

  • 使用最小蛋白质 (15-60μl) 快速相位边界映射.
  • 核化区和特定形态区域的识别.
  • 对于核化有限系统,对元稳定区的精确定义.

结论:

  • 综合方法系统地将核化与生长脱而出.
  • 确保稳定的化学坐标扩大规模,与蒸汽扩散不同.
  • 将实证样品准备转化为连续衍射的合理,高效的过程.