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使用cIMS-MS/MS识别GOS二糖化异构体

Bram van de Put¹, Cindy Steven¹, Carolina O Pandeirada¹

¹Laboratory of Food Chemistry, Wageningen University, Bornse Weilanden 9, Wageningen 6708 WG, The Netherlands.

Journal of the American Society for Mass Spectrometry

|January 27, 2026

概括

使用添加剂的循环离子移动性质谱 (cIMS-MS) 有效地分离和识别异构分糖. 这种方法简化了寡糖类分析,通过从漂移时间推断组成,从碎片化模式推断联系.

科学领域:

分析化学分析化学
碳水化合物化学碳水化合物化学
质谱测量质量谱测量

背景情况:

在复杂的混合物中表征异构性寡糖类,如银河寡糖类 (GOS),由于大量的可能结构,这 presents 提出了重大的分析挑战.
结构可能性的指数级增长与更高的聚合度 (DP) 使得获得完整的分析标准很困难.

研究的目的:

调查循环离子移动性质谱 (cIMS) 和碰撞诱导解离 (CID) 碎片化的联合能力,以识别异构分糖.
评估不同金属添加物 (,,,,,,) 在cIMS分离和CID碎片化中对银和基于葡萄糖的二糖的性能.

主要方法:

研究了使用cIMS和CID组合的同位素二糖的分离和碎片化.
对比了,,,卢比和添加物的漂移时间选择性和碎片产量.
分析了cIMS漂移时间和CID碎片化模式与分糖化合物组成和链接类型的相关性.

主要成果:

与其他金属离子相比,添加物在cIMS中表现出卓越的性能,提供了良好的分辨率和显著更高的碎片产量.
cIMS漂移时间主要受二糖化合物组成的影响,在相同的链接类型中始终显示Gal-Gal < Gal-Glc < Glc-Glc的顺序.
证实CID交叉环碎片化模式是特定于连接类型的,对组合或异构配置的依赖最小.

结论:

开发的cIMS-MS/MS方法通过从CID碎片化中确定漂移时间和链接类型的单糖化合物组成,可以直接识别未知的二糖化合物.
这种方法减少了对更高DP寡糖标准的需求,为分析复杂寡糖混合物提供了一条途径.
这些发现代表了使用基于cIMS-MS的技术识别较大的寡糖的关键进步.

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