相关实验视频

Updated: Jan 28, 2026

Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain

Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain

Published on: July 11, 2016

细菌细菌RK027通过基因表达调节诱导米中的应激耐受性

Abu Barkat Md Gulzar¹, Pranab Behari Mazumder¹

¹Department of Biotechnology, Assam University, Silchar, Assam, India.

Journal of basic microbiology

|January 27, 2026

概括

细菌细菌RK027通过促进植物防御基因,在压力下增强大米生长. 这种促进植物生长的根茎细菌 (PGPR) 提高了植物对酸盐的耐受性,为受污染的农田提供了解决方案.

科学领域:

环境微生物学环境微生物学
植物与微生物的相互作用
生物技术是生物技术.

背景情况:

农业中的污染严重阻碍了植物生长和作物产量.
促进植物生长的草根细菌 (PGPR) 可以减轻植物压力,但它们诱导系统性耐受性 (IST) 的机制尚未完全理解.
了解这些机制对于制定改善污染环境中的作物弹性策略至关重要.

研究的目的:

从大米根球中分离出耐的PGPR.
为了评估一个孤立的PGPR,Bacillus subtilis RK027的有效性,在促进矿 (As^III) 压力下的米生长.
阐明由B. subtilis RK027介导的IST的分子机制,重点关注斯蒙酸 (JA) 和抗氧化剂基因表达.

主要方法:

从大米根球中分离和识别耐的PGPR.
在压力下对PGPR特征 (IAA,氨,EPS产量,酸盐溶解,ACCD活性) 的表征.
里埃变换红外光谱法 (FT-IR) 对外聚糖 (EPS) 的分析.
在注射后对大米植物形态生理和生化特征的评估.
定量实时PCR (qRT-PCR) 分析JA生物合成,信号和抗氧化基因的表达.

主要成果:

鉴定出Bacillus subtilis RK027,一种耐的PGPR,并显示出多种植物生长促进 (PGP) 特性.
由RK027产生的外聚糖 (EPS) 可能与AsIII形成复合体,减少其在植物中的转移.
接种RK027的水植物在A^III压力下显示出生长,生理和抗氧化活性的显著改善.
RK027显著上调关键基因,这些基因参与了斯酸 (JA) 生物合成 (OsAOS1),信号传递 (OsJAR1) 和抗氧化防御 (OsSOD-Cu/Zn,OsCATA,OsAPX1).

结论:

细菌细菌RK027有效促进大米的生长,并提高对酸盐压力的耐受性.
IST机制涉及增强的JA生物合成和下游抗氧化途径,由B. subtilis RK027.2介导.
B. subtilis RK027具有显著的潜力,可以改善污染的农业土壤中的大米种植.

关键词:

B.subtilis RK02727 在这里可以使用.这就是它,它就是它.米树 (Oryzae sativa L. L.) 是一种植物.抗氧化酶是一种抗氧化酶酶.的压力压力是.基因表达的基因表达方式斯酸是一种的酸.种子初始化种子初始化

更多相关视频

Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores

Visualization of Germinosomes and the Inner Membrane in Bacillus subtilis Spores

Published on: April 15, 2019

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Published on: June 30, 2023

相关概念视频

Constitutive and Regulated Gene Expression

Constitutive and Regulated Gene Expression

Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...

What is Gene Expression?

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...

Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Chromatin Position Affects Gene Expression

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...

Responses to Salt Stress

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.