吸烟对慢性牙周炎中牙下微生物群的影响:一项16S rRNA测序研究
1Periodontology Department, Faculty of Dentistry, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Dentistry journal
|January 27, 2026
概括
沉重的吸烟显著降低了口腔微生物群的多样性,并增加了有害细菌,如Fusobacterium在牙周炎患者. 适度吸烟也会改变细菌的组成,强调吸烟.
科学领域:
- 口腔微生物学 口腔微生物学
- 牙周病学 牙周病学
- 微生物生态学 微生物生态学
背景情况:
- 吸烟会对牙周健康产生负面影响.
- 吸烟强度是牙周炎分级的一个因素.
- 吸烟强度对下微生物群的影响需要进行更深入的研究.
研究的目的:
- 根据吸烟强度,评估慢性牙周炎患者下微生物组的差异.
- 为了比较重度吸烟者 (≥10支/天),中度吸烟者 (<10支/天) 和非吸烟者.
主要方法:
- 60名慢性牙周炎患者的横截面分析研究.
- 从牙周口袋收集的下斑块样本.
- 16S rRNA测序 (Ion Torrent S5) 用于分析微生物社区结构和多样性 (Shannon,Simpson,Chao指数).
主要成果:
- 下细菌多样性在重度吸烟者中最低,在非吸烟者中最高.
- 特定的细菌 (Veillonella,Streptococcus,Prevotella,Fusobacterium,Dialister) 在不同组的患病率不同.
- 增加的吸烟强度与减少的Campylobacter和增加的Fusobacterium丰度相关;中度吸烟者具有更高的Spirochaetes.
结论:
- 更高的吸烟强度与增加的Fusobacterium丰富性和减少的下微生物群多样性有关.
- 这表明,在重度吸烟者的下口袋中,细菌环境更稳定.
- 这些发现强调了吸烟强度对牙周炎口腔微生物群的影响.
相关概念视频
Gene Evolution - Fast or Slow?
8.2K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
8.2K
Cis-regulatory Sequences
11.7K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.7K
Chronic Obstructive Pulmonary Disease-IV: Assessement and Diagnostic Studies
3.1K
Assessing and diagnosing Chronic Obstructive Pulmonary Disease (COPD) involves a detailed approach that includes a comprehensive review of medical history, physical examination, and a variety of diagnostic tests. This thorough evaluation is essential to ensure an accurate diagnosis and guide effective management strategies.
Medical History
Medical History
3.1K
Sanger Sequencing
774.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
774.3K
Chronic Pharyngitis
11.6K
Chronic pharyngitis refers to persistent inflammation of the pharyngial mucosa.
Etiology
It often arises from persistent viral or bacterial infections affecting sinuses and tonsils.
Additional contributing factors include inadequate dental hygiene, mouth breathing, recurring tonsillitis, allergic rhinitis, laryngopharyngeal reflux, and exposure to smoke, chemicals, and other environmental pollutants. Allergic reactions to pollen, mold, and pet dander, chronic cough, excessive voice usage,...
Etiology
It often arises from persistent viral or bacterial infections affecting sinuses and tonsils.
Additional contributing factors include inadequate dental hygiene, mouth breathing, recurring tonsillitis, allergic rhinitis, laryngopharyngeal reflux, and exposure to smoke, chemicals, and other environmental pollutants. Allergic reactions to pollen, mold, and pet dander, chronic cough, excessive voice usage,...
11.6K
Next-generation Sequencing
98.4K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
98.4K


