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不同粗度的和植入物表面对口腔上皮细胞的影响
Marco Aoqi Rausch1,2,3, Zhiwei Tian1, Vera Maierhofer1
1Competence Center for Periodontal Research, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, Austria.
Dentistry journal
|January 27, 2026
概括
牙植入物表面粗度,而不是材料,影响口腔上皮细胞的行为. 更粗的表面促进细胞增殖和增强屏障功能,可能改善周围植入物组织的整合.
科学领域:
- 生物材料科学 生物材料科学
- 口腔生物学 口腔生物学
- 细胞生物学 细胞生物学
背景情况:
- 在植入物学中,实现口腔软组织与牙科植入物紧密集成至关重要.
- 口腔上皮细胞在这种软组织整合过程中发挥着关键作用.
- 了解植入物表面特征如何影响这些细胞至关重要.
研究的目的:
- 在体外研究不同粗度的和的表面对口腔上皮细胞参数的影响.
- 为了比较加工 (光滑) 与喷砂和酸蚀刻 (中等粗) 表面的影响.
主要方法:
- 在机械加工的 (TiM),机械加工的 (ZrM),喷砂/酸蚀刻的 (SLA) 和喷砂/酸蚀刻的 (ZLA) 表面上培养了人类口腔状癌Ca9-22细胞.
- 评估细胞增殖/活力 (CCK-8),细胞形态 (光显微镜).
- 对IL-8,ICAM-1,E-cadherin,ITG-α6,ITG-β4和IL-8蛋白质水平 (ELISA) 的量化基因表达 (qPCR) 测试.
主要成果:
- 与加工表面相比,更粗的表面 (SLA,ZLA) 促进了初始细胞的增殖.
- 表面类型之间注意到细胞附着的差异.
- 较粗的表面显著上调整整合素 (ITG) -α,ITG-β4和细胞间粘附分子 (ICAM) -1的表达.
- 机械加工的表面显示了略高的互白素 (IL) - 8水平.
- 没有观察到表面材料 (与) 的显著影响.
结论:
- 口腔上皮细胞的特性,包括免疫和屏障功能,受到植入物表面粗度的适度影响,但不是材料本身.
- 表面粗度可能在确定植入周围表皮质的质量方面发挥作用.
- 这些发现表明,表面地形是优化牙科植入物周围软组织整合的关键因素.
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