对细胞路微流体CTC丰富和识别平台的评估,用于转移性NSCLC中CTC检测
Ebru Özgür1, Ayça Çırçır1, Begüm Şen Doğan1
1Mikro Biyosistemler A.S., 06530 Ankara, Türkiye.
Biosensors
|January 27, 2026
概括
一个新的Cellsway微流体平台有效地将循环瘤细胞 (CTC) 从非小细胞肺癌 (NSCLC) 患者中隔离出来,使用无标签丰富. 这种微侵袭的液体活检方法显示出高特异性和恢复率,可用于实时疾病监测.
科学领域:
- 在瘤学瘤学.
- 生物技术是生物技术.
- 医学诊断 医学诊断 医学诊断
背景情况:
- 肺癌是全球癌症死亡的主要原因之一.
- 非小细胞肺癌 (NSCLC) 占大多数肺癌病例.
- 传统的组织活检是侵入性的,并且限制了重复监测.
研究的目的:
- 开发和验证一种新型的微流体平台,用于有效和可重复的循环瘤细胞 (CTC) 隔离.
- 通过液体活检实现非小细胞肺癌 (NSCLC) 的最小侵入性实时监测.
主要方法:
- 开发Cellsway微流体平台,利用惯性水力动力学和螺旋道与翼柱为无标签CTC丰富.
- 通过使用NSCLC细胞系 (H1975和A549) 的增实验进行分析验证.
- 临床验证来自51名IV期NSCLC患者的血液样本,每样处理7.5毫升血液.
主要成果:
- 高回收率:在分析验证中,H1975细胞的91.9%,A549细胞的78.3%.
- 在47%的第四阶段NSCLC患者中检测到CTC,每7.5毫升血液中数量从0到72个CTC.
- 95%的高特异性在每7.5毫升1个CTC的切割值,有效丰富患者衍生的CTC.
结论:
- 塞尔斯威平台提供高效,可复制和高度特定的CTC隔离.
- 这项技术在临床监测和推进NSCLC精度瘤学方面具有重大潜力.
- 使用惯性水力动力学的无标签丰富对于隔离CTC,甚至是较小的患者衍生的CTC是有效的.
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