芯片上器官的微流体室设计:一个计算流体动力学研究柱体几何和脉冲性 perfusion 的研究
Andi Liao1,2, Jiwen Xiong1,2,3, Zhirong Tong1,2,3
1Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China.
Biosensors
|January 27, 2026
概括
器官在芯片 (OOC) 平台从优化的室内设计中受益. 在OOC设备中的柱状腔改善了细胞剪切应力均性,减少了停滞的流量,增强了对心血管研究的生理相关性.
科学领域:
- 生物医学工程 生物医学工程
- 微流体学 微流体学
- 细胞生理学 细胞生理学
背景情况:
- 器官在芯片 (OOC) 平台模仿人体器官生理在体外.
- 流体力学显著影响OOC系统中的细胞行为,特别是用于心血管应用.
- 现有的OOC设计通常使用简单的几何和稳定的流动,导致非均的细胞刺激.
研究的目的:
- 研究室几何和流入条件对OOC平台近壁流环境的影响.
- 为了比较平面与柱状室在脉冲性 perfusion 下的流体动力学性能.
- 为生理相关的细胞培养优化OOC微环境.
主要方法:
- 使用了计算流体动力学 (CFD) 模拟.
- 分析近壁流量特征,包括相对停留时间 (RRT) 和时间平均壁切削应力 (TAWSS).
- 不同的室内几何形状 (平面与柱状) 和流入条件 (稳定与脉动) 的比较.
主要成果:
- 与平面室相比,柱状室显著减少了相对居住时间.
- 小支柱配置产生了最均的TAWSS分布.
- 墙壁剪切应力在脉动性流动下表现出相位依赖的变化,突出了稳定流入假设的局限性.
结论:
- 室内几何和流入条件极大地影响了OOC平台中的微环境.
- 支柱式设计可以更好地控制剪切应力和流动模式.
- 这些发现为开发更准确,更可靠的OOC模型提供了指导,用于基于细胞的研究.
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