用CRISPR/Cas9进行基因编辑的单细胞菌株Ganoderma sichuanense的查
Le Li1, Yuxuan Liu1, Jianzhong Wu1
1College of Mycology, Jilin Agricultural University, Changchun 130118, China.
Journal of fungi (Basel, Switzerland)
|January 27, 2026
概括
这项研究开发了Ganoderma sichuanense的CRISPR/Cas9基因转换平台,确定了基因编辑和功能研究的最佳菌株. 这个平台的平台的平台.
科学领域:
- 菌类学 菌类学是指菌类学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 甘菌 (Ganoderma sichuanense) 是一种有价值的药用真菌,具有显著的遗传多样性.
- 现有的基因转换系统在不同的G. sichuanense基因型中可能不会普遍有效.
- 缺乏系统的评估阻碍了该物种的功能遗传研究.
研究的目的:
- 为了评估跨多种Ganoderma sichuanense基因型的基因转换系统的效率.
- 建立一个强大的CRISPR/Cas9平台,用于高效的基因编辑G. sichuanense.
- 为未来的遗传改进和功能研究确定最佳菌株.
主要方法:
- 选八个G.sichuanense菌株,以检测它们对乙基菌素的耐药性和GFP的表达.
- 通过PEG介导的原生质细胞转化来评估基因表达效率.
- 开发和应用双sgRNACRISPR/Cas9系统,针对 ura3 基因和五个功能基因.
主要成果:
- 三种菌株 (CCMJ1500101,CCMJ1509001,CCMJ1507802) 显示了高的转化效率 (20.0-66.7%).
- 克里斯普尔/卡斯9基因编辑效率因基因型而异 (14.3-75.0%),表明基因型依赖.
- 在菌株CCMJ1500101中成功编辑了五个功能基因,并检测了新的基因逆转事件.
结论:
- 已经建立了一个强大的双sgRNACRISPR/Cas9平台,用于G. sichuanense.
- 菌株CCMJ1500101被确定为基因操纵的高效资源.
- 这项工作促进了未来的基因功能研究和G. sichuanense的遗传改进.
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